Chiesa Oscar Alberto, von Bredow Jurgen, Smith Michelle, Thomas Michael
Center for Veterinary Medicine, Office of Research, Division of Residue Chemistry, Food and Drug Administration, 8401 Muirkirk Road, Laurel, MD 20708, USA.
Res Vet Sci. 2009 Aug;87(1):133-4. doi: 10.1016/j.rvsc.2008.10.016. Epub 2008 Dec 9.
The purpose of this study was to simplify the two-port laparoscopic renal biopsy technique used in support of pharmacokinetic studies through the application of a one-port system. Twelve Holstein steers were fasted for 24 h and sedated with acepromazine and xylaxine in preparation for laparoscopic surgery in standing stocks. Lidocaine 2% was injected to provide local anesthesia for introduction of the trocar-cannula assembly. The operating endoscope was inserted and the abdomen was insufflated with CO(2). A biopsy forceps was introduced into the channel of the operating endoscope to obtain a 100mg kidney cortex sample. Eighteen laparoscopic procedures provided 18 kidney samples suitable for pharmacokinetic studies. No complications were encountered. The one-port laparoscopic kidney biopsy is feasible and safe, and advanced skill required for triangulation is not necessary for its performance.
本研究的目的是通过应用单孔系统简化用于支持药代动力学研究的两孔腹腔镜肾活检技术。12头荷斯坦奶牛禁食24小时，并用乙酰丙嗪和赛拉嗪镇静，为站立式畜栏中的腹腔镜手术做准备。注射2%利多卡因以提供局部麻醉，用于插入套管针-套管组件。插入手术内窥镜，用二氧化碳对腹部进行充气。将活检钳插入手术内窥镜的通道以获取100mg肾皮质样本。18例腹腔镜手术提供了18个适合药代动力学研究的肾脏样本。未出现并发症。单孔腹腔镜肾活检可行且安全，其操作不需要三角测量所需的高级技能。
