Tang Shuang, Wang Yongsheng, Zhang Dong, Gao Yajun, Ma Yefei, Yin Baoying, Sun Jianhong, Liu Jun, Zhang Yong
Institute of Biotechnology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Anim Reprod Sci. 2009 Oct;115(1-4):1-9. doi: 10.1016/j.anireprosci.2008.10.018. Epub 2008 Oct 31.
This study investigated the effects of donor cells pretreated with oocyte extracts on in vitro development of cloned embryos. Bovine fibroblasts were exposed to immature, mature and parthenogenetic oocyte extracts respectively before nuclear transfer. The detectable expression of Oct4 and global deacetylation in the treated cells showed that extracts could reprogram fibroblasts. Although all three groups of extracts exhibited reprogramming capacity, embryo development was not compliant with reprogramming effect. Improved quality and development of blastocysts were observed only in the mature extract treated group. We demonstrated that pretreatment of donor cells with mature oocyte extract improved in vitro development of cloned embryos. Our results suggested that reprogramming donor nuclei to a state synchronized with recipient cytoplasm before nuclear transfer would be beneficial for the development of cloned embryos.
本研究调查了经卵母细胞提取物预处理的供体细胞对克隆胚胎体外发育的影响。在进行核移植之前，分别将牛成纤维细胞暴露于未成熟、成熟和孤雌生殖的卵母细胞提取物中。处理后的细胞中Oct4的可检测表达和整体去乙酰化表明提取物可以对成纤维细胞进行重编程。尽管三组提取物均表现出重编程能力，但胚胎发育与重编程效果并不一致。仅在成熟提取物处理组中观察到囊胚质量和发育的改善。我们证明，用成熟卵母细胞提取物预处理供体细胞可改善克隆胚胎的体外发育。我们的结果表明，在核移植前将供体细胞核重编程到与受体细胞质同步的状态将有利于克隆胚胎的发育。
