Cainé Laura M, Real Francisco Corte, Saloña-Bordas Marta I, de Pancorbo M Martínez, Lima Gabiela, Magalhães Teresa, Pinheiro Fátima
Instituto Nacional Medicina Legal, Portugal.
Forensic Sci Int. 2009 Jan 30;184(1-3):e21-3. doi: 10.1016/j.forsciint.2008.10.016. Epub 2008 Dec 9.
Medico-legal entomology, one area in the broad field of entomology, is routinely used in forensic applications. Insects are often collected from a corpse during criminal information related to the body, but requires the fast and accurate identification of the species attracted to the remains. The local entomofauna in most cases is important for explaining entomological evidence. The survey of the local entomofauna has become a fundamental first step in forensic entomological studies, because different geographical distributions, seasonal and environmental factors may influence the decomposition process and the occurrence of different species on corpses. A morphological and DNA-based methods for species identification were used in this study. Thirty-two cases are reported from indoors and outdoors conditions. Specimens were collected from corpses during autopsy procedures in the National Institute of Legal Medicine, Portugal, and cases were summarized by sex, death local, month of discovery, probable cause of death, species found and number of analyzed specimens. Just eight species, mainly Calliphoridae together with one Sarcophagidae were reported from corpses. The DNA sequencing was performed to study the mitochondrial encoded subunit I of the cytochrome oxidase gene. The aim of this work was the beginning of a database of the cadaveric entomofauna in Portugal.
法医昆虫学是昆虫学广泛领域中的一个领域，常用于法医应用。在与尸体相关的刑事调查中，常从尸体上收集昆虫，但这需要快速准确地识别被尸体吸引的物种。在大多数情况下，当地的昆虫区系对于解释昆虫学证据很重要。对当地昆虫区系的调查已成为法医昆虫学研究的基本第一步，因为不同的地理分布、季节和环境因素可能会影响尸体的分解过程以及不同物种在尸体上的出现情况。本研究采用了基于形态学和DNA的物种鉴定方法。报告了32例室内和室外条件下的案例。标本是在葡萄牙国家法医学研究所的尸检过程中从尸体上收集的，并按性别、死亡地点、发现月份、可能的死亡原因、发现的物种和分析标本数量对案例进行了总结。从尸体上仅报告了8个物种，主要是丽蝇科以及1个麻蝇科。进行了DNA测序以研究细胞色素氧化酶基因的线粒体编码亚基I。这项工作的目的是建立葡萄牙尸体昆虫区系数据库的开端。
