Sonet Gontran, Jordaens Kurt, Braet Yves, Bourguignon Luc, Dupont Eréna, Backeljau Thierry, De Meyer Marc, Desmyter Stijn
Royal Belgian Institute of Natural Sciences, OD Taxonomy and Phylogeny (JEMU), Vautierstraat 29, 1000 Brussels, Belgium.
Royal Museum for Central Africa, Department of Biology (JEMU), Leuvensesteenweg 13, 3080 Tervuren, Belgium ; University of Antwerp, Evolutionary Ecology Group, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
Zookeys. 2013 Dec 30(365):307-28. doi: 10.3897/zookeys.365.6027.
Fly larvae living on dead corpses can be used to estimate post-mortem intervals. The identification of these flies is decisive in forensic casework and can be facilitated by using DNA barcodes provided that a representative and comprehensive reference library of DNA barcodes is available. We constructed a local (Belgium and France) reference library of 85 sequences of the COI DNA barcode fragment (mitochondrial cytochrome c oxidase subunit I gene), from 16 fly species of forensic interest (Calliphoridae, Muscidae, Fanniidae). This library was then used to evaluate the ability of two public libraries (GenBank and the Barcode of Life Data Systems - BOLD) to identify specimens from Belgian and French forensic cases. The public libraries indeed allow a correct identification of most specimens. Yet, some of the identifications remain ambiguous and some forensically important fly species are not, or insufficiently, represented in the reference libraries. Several search options offered by GenBank and BOLD can be used to further improve the identifications obtained from both libraries using DNA barcodes.
寄生于尸体上的蝇幼虫可用于估计死后间隔时间。在法医案件工作中，对这些苍蝇的鉴定至关重要，并且如果有一个具有代表性和综合性的DNA条形码参考文库，利用DNA条形码可以促进鉴定工作。我们构建了一个本地（比利时和法国）参考文库，其中包含来自16种具有法医意义的苍蝇物种（丽蝇科、蝇科、厕蝇科）的85条细胞色素c氧化酶亚基I基因（COI DNA条形码片段，线粒体）序列。然后，该文库被用于评估两个公共文库（GenBank和生命条形码数据系统 - BOLD）对来自比利时和法国法医案件标本的鉴定能力。公共文库确实能够正确鉴定大多数标本。然而，一些鉴定结果仍然不明确，并且一些在法医上很重要的苍蝇物种在参考文库中没有或没有充分体现。GenBank和BOLD提供的几种搜索选项可用于进一步改进从这两个文库使用DNA条形码获得的鉴定结果。
