Grant D, Hurlbut D, Zhong R, Wang P Z, Chen H F, Garcia B, Behme R, Stiller C, Duff J
Department of Microbiology, University of Western Ontario, Canada.
Transplantation. 1991 Aug;52(2):221-4. doi: 10.1097/00007890-199108000-00007.
In addition to its role in absorbing nutrients, the intestinal mucosa provides an important barrier against toxins and bacteria in the bowel lumen. The present study evaluated gut barrier function following orthotopic (in continuity) intestinal grafting in rats. Graft histology, intestinal permeability, and bacterial translocation to the grafted mesenteric lymph nodes, the host's liver, and the host's spleen were assessed on the 3rd, 5th, and 7th postoperative days. The study group received no immunosuppression after allotransplantation. The two control groups included rats with isografts and rats with cyclosporine-treated allografts. On the 7th POD, the study animals had moderate transmural inflammation due to rejection, with normal histology in the isografts and CsA-treated allografts; increased intestinal permeability, measured by urinary excretion of oral 51Cr-EDTA (P less than 0.01); and increased number of bacteria in the MLN and spleen (P less than 0.05). The number of bacteria in the MLN and spleen of the study group positively correlated with the changes in intestinal permeability (P less than 0.05). Rejection of the orthotopic intestinal graft leads to increased intestinal permeability and bacterial translocation from the lumen of the graft to the host's reticuloendothelial system. Measures to improve gut barrier function and antibiotic therapy during rejection episodes may help reduce the incidence of septic complications after intestinal grafting.
除了在吸收营养方面发挥作用外，肠黏膜还为抵御肠腔内的毒素和细菌提供了重要屏障。本研究评估了大鼠原位（连续）肠道移植后的肠道屏障功能。在术后第3天、第5天和第7天评估移植组织学、肠道通透性以及细菌向移植的肠系膜淋巴结、宿主肝脏和宿主脾脏的移位情况。研究组在同种异体移植后未接受免疫抑制。两个对照组包括同基因移植大鼠和环孢素处理的同种异体移植大鼠。在术后第7天，研究动物因排斥反应出现中度透壁性炎症，同基因移植组和环孢素处理的同种异体移植组组织学正常；口服51Cr - EDTA经尿液排泄测定的肠道通透性增加（P < 0.01）；肠系膜淋巴结和脾脏中的细菌数量增加（P < 0.05）。研究组肠系膜淋巴结和脾脏中的细菌数量与肠道通透性变化呈正相关（P < 0.05）。原位肠道移植的排斥反应导致肠道通透性增加以及细菌从移植肠腔向宿主网状内皮系统移位。在排斥反应期间改善肠道屏障功能的措施和抗生素治疗可能有助于降低肠道移植后败血症并发症的发生率。
