Bozzaro Salvatore, Bucci Cecilia, Steinert Michael
Department of Clinical and Biological Sciences, University of Turin, Ospedale S. Luigi, 10043 Orbassano, Italy.
Int Rev Cell Mol Biol. 2008;271:253-300. doi: 10.1016/S1937-6448(08)01206-9.
Research into phagocytosis and host-pathogen interactions in the lower eukaryote Dictyostelium discoideum has flourished in recent years. This chapter presents a glimpse of where this research stands, with emphasis on the cell biology of the phagocytic process and on the wealth of molecular genetic data that have been gathered. The basic mechanistic machinery and most of the underlying genes appear to be evolutionarily conserved, reflecting the fact that phagocytosis arose as an efficient way to ingest food in single protozoan cells devoid of a rigid cell wall. In spite of some differences, the signal transduction pathways regulating phagosome biogenesis are also emerging as ultimately similar between Dictyostelium and macrophages. Both cell types are hosts for many pathogenic invasive bacteria, which exploit phagocytosis to grow intracellularly. We present an overwiew, based on the analysis of mutants, on how Dictyostelium contributes as a genetic model system to decipher the complexity of host-pathogen interactions.
近年来，对低等真核生物盘基网柄菌吞噬作用及宿主 - 病原体相互作用的研究蓬勃发展。本章简要介绍了该研究的现状，重点关注吞噬过程的细胞生物学以及已收集到的大量分子遗传学数据。基本的机制和大多数相关基因似乎在进化上是保守的，这反映出吞噬作用最初是单细胞原生动物摄取食物的一种有效方式，这些原生动物没有坚硬的细胞壁。尽管存在一些差异，但在盘基网柄菌和巨噬细胞之间，调节吞噬体生物发生的信号转导途径最终也显示出相似性。这两种细胞类型都是许多致病性入侵细菌的宿主，这些细菌利用吞噬作用在细胞内生长。基于对突变体的分析，我们概述了盘基网柄菌作为一种遗传模型系统如何有助于解读宿主 - 病原体相互作用的复杂性。
