用温度敏感型铜绿假单胞菌对小鼠进行口服免疫可增强野生型菌株的肺部清除能力。

Oral immunization of mice with temperature-sensitive Pseudomonas aeruginosa enhances pulmonary clearance of the wild-type.

作者信息

Hooke A M, Cerquetti M C, Wan K S, Wang Z, Sordelli D O, Bellanti J A

机构信息

Department of Pediatrics, Georgetown University School of Medicine, Washington, DC 20007.

出版信息

Vaccine. 1991 May;9(5):294-6. doi: 10.1016/0264-410x(91)90052-8.

DOI:10.1016/0264-410x(91)90052-8

PMID:1908162

Abstract

DBA/2J mice were immunized daily for 3 days per os with 10(8)-10(9) colony forming units (c.f.u.) of two different temperature-sensitive (TS) mutants of Pseudomonas aeruginosa. At varying times after the final immunization the animals were exposed to aerosols of the parental immunotype 1, and the ability of the immunized and control mice to clear their lungs of the wild-type (WT) challenge was measured 4 h later. The number of c.f.u. remaining in the lungs of mice immunized with one mutant, D/1/8, was significantly less (p less than 0.01) than the number remaining in the lungs of control mice and mice immunized with a second TS mutant, E/9/9.

摘要

用10⁸ - 10⁹个菌落形成单位（c.f.u.）的两种不同温度敏感（TS）铜绿假单胞菌突变体，经口对DBA/2J小鼠进行每日一次，共3天的免疫。在最后一次免疫后的不同时间，将动物暴露于亲本免疫型1的气溶胶中，并在4小时后测量免疫小鼠和对照小鼠清除其肺部野生型（WT）攻击的能力。用一种突变体D/1/8免疫的小鼠肺部残留的c.f.u.数量，显著少于（p < 0.01）对照小鼠和用第二种TS突变体E/9/9免疫的小鼠肺部残留的数量。