Casslén B G, Harper M J
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio.
Acta Endocrinol (Copenh). 1991 Jul;125(1):101-8. doi: 10.1530/acta.0.1250101.
The aim of the study was to explore the possibility of using human endometrial epithelial cells in serum-free culture as a sensitive assay for hormonal effects on the human endometrium. Glands were isolated following enzymatic digestion of the endometrial tissue and plated on a collagen matrix. The epithelial cells were grown in either medium containing serum or in supplemented serum-free medium. No morphologic difference was found between cells grown in these two media for up to 5 days, using either light or scanning electron microscopy. Secretion of prostaglandin F2 alpha (PGF2 alpha) in response to estradiol was not lower in serum-free medium than in medium containing serum for the first 2 days of culture, whereas secretion declined after prolonged incubation in the serum-free medium. This response to estradiol was clearly dose-dependent, and it was further enhanced by addition of arachidonic acid, the precursor for prostaglandin synthesis, to the medium. Co-culture of endometrial stromal cells did not influence the secretion of PGF2 alpha by epithelial cells. We conclude that the secretion of PGF2 alpha from primary cultures of human endometrial epithelial cells grown on collagen in serum-free medium can be used for a limited period as an assay of estrogenic effects on the human endometrium.
本研究的目的是探索将人子宫内膜上皮细胞在无血清培养中作为一种检测激素对人子宫内膜影响的敏感方法的可能性。在对子宫内膜组织进行酶消化后分离腺体，并接种于胶原基质上。上皮细胞在含血清培养基或补充的无血清培养基中生长。使用光学显微镜或扫描电子显微镜观察发现，在这两种培养基中生长长达5天的细胞之间没有形态学差异。在培养的前两天，无血清培养基中前列腺素F2α（PGF2α）对雌二醇的反应分泌并不低于含血清培养基，而在无血清培养基中长时间孵育后分泌下降。这种对雌二醇的反应明显呈剂量依赖性，并且通过向培养基中添加前列腺素合成前体花生四烯酸进一步增强。子宫内膜基质细胞的共培养不影响上皮细胞PGF2α的分泌。我们得出结论，在无血清培养基中胶原上生长的人子宫内膜上皮细胞原代培养物中PGF2α的分泌可在有限时间内用作检测雌激素对人子宫内膜影响的方法。
