DNA的组成分析方法。
Approaches to the compositional analysis of DNA.
作者信息
Manderville Richard A, Kropinski Andrew M
机构信息
Department of Chemistry, University of Guelph, Guelph, Ontario, Canada.
出版信息
Methods Mol Biol. 2009;502:11-7. doi: 10.1007/978-1-60327-565-1_2.
PMID:19082548
Abstract
DNA base compositional analysis is something which is rarely undertaken today, but it is still a useful criterion for phage taxonomy. A variety of techniques are described including hydrolysis of the DNA to the level of bases or nucleosides and separation by paper chromatography or HPLC. Spectroscopic and spectrofluorometric procedures are also outlined.
摘要
DNA碱基组成分析如今很少进行，但它仍是噬菌体分类学的一个有用标准。文中描述了多种技术，包括将DNA水解到碱基或核苷水平，并通过纸色谱法或高效液相色谱法进行分离。还概述了光谱和荧光光谱程序。
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