从天然冬虫夏草中分离出的真菌菌株的随机扩增多态性DNA（RAPD）分析及核苷评估。

Random amplified polymorphic DNA (RAPD) analysis and the nucleosides assessment of fungal strains isolated from natural Cordyceps sinensis.

作者信息

Feng K, Wang S, Hu D J, Yang F Q, Wang H X, Li S P

机构信息

Institute of Chinese Medical Sciences, University of Macau, Macao.

出版信息

J Pharm Biomed Anal. 2009 Oct 15;50(3):522-6. doi: 10.1016/j.jpba.2009.04.029. Epub 2009 May 5.

DOI:10.1016/j.jpba.2009.04.029

PMID:19481405

Abstract

Random amplified polymorphic DNA (RAPD) and high performance liquid chromatography (HPLC) were applied to investigate genetic and chemical variations of 2 natural C. sinensis, 16 fungal strains isolated from C. sinensis, and 2 fungal strains of C. militaris. Five of the 68 arbitrary decamer primers were available for discrimination of the investigated samples. As a result, 20 investigated samples were divided into three main clusters according to the genetic distance, and some fungal strains isolated from natural C. sinensis were obviously different. But according to the contents of nucleosides, including uracil, uridine, hypoxanthine, inosine, guanosine, adenosine, adenine, and cordycepin, natural and cultured Cordyceps were in two individual sub-groups, which suggested that chemical characteristics among cultured mycelia of different fungal strains isolated from natural C. sinensis were similar, but they were different from natural one.

摘要

应用随机扩增多态性DNA（RAPD）和高效液相色谱法（HPLC）研究了2种天然中华虫草、从中华虫草中分离出的16株真菌菌株以及2株蛹虫草菌株的遗传和化学变异。68个随机十聚体引物中有5个可用于区分所研究的样本。结果，根据遗传距离将20个研究样本分为三个主要聚类，从天然中华虫草中分离出的一些真菌菌株明显不同。但根据核苷含量，包括尿嘧啶、尿苷、次黄嘌呤、肌苷、鸟苷、腺苷、腺嘌呤和虫草素，天然和培养的虫草分为两个单独的亚组，这表明从天然中华虫草中分离出的不同真菌菌株的培养菌丝体之间的化学特征相似，但与天然虫草不同。