通过脉冲场凝胶电泳测定噬菌体基因组大小。
Determination of bacteriophage genome size by pulsed-field gel electrophoresis.
作者信息
Lingohr Erika, Frost Shelley, Johnson Roger P
机构信息
Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.
出版信息
Methods Mol Biol. 2009;502:19-25. doi: 10.1007/978-1-60327-565-1_3.
PMID:19082549
Abstract
Standard agarose gel electrophoresis is extensively used to resolve DNA fragments from 0.2 to 40-50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb.
摘要
标准琼脂糖凝胶电泳被广泛用于分离大小在0.2至40 - 50 kb之间的DNA片段。基因组DNA的较大片段或整个病毒基因组只能通过脉冲场凝胶电泳（PFGE）有效分离，该技术将分子分离范围扩展至200 bp至12 Mb。
Zotero 插件