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基于计算机模拟对DNA基序进行表征,特别涉及启动子和终止子。

In silico characterization of DNA motifs with particular reference to promoters and terminators.

作者信息

Lavigne Rob, Villegas André, Kropinksi Andrew M

机构信息

Department of Biosystems, Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.

出版信息

Methods Mol Biol. 2009;502:113-29. doi: 10.1007/978-1-60327-565-1_8.

Abstract

Knowledge of the regulatory elements contained within bacteriophage genomes forms the basis for understanding genomic expression and organization. The in silico prediction of promoter and terminator sequences in phage genomes is a first step towards this understanding. In this chapter, a number of programs and resources to identify regulatory elements are listed and discussed. Combining the available web-resources and literature data optimizes these predictions and can thus aid in a more directed experimental identification of these regulatory elements.

摘要

了解噬菌体基因组中所含的调控元件是理解基因组表达和组织的基础。对噬菌体基因组中的启动子和终止子序列进行计算机预测是迈向这一理解的第一步。在本章中,列出并讨论了一些用于识别调控元件的程序和资源。结合现有的网络资源和文献数据可优化这些预测,从而有助于更有针对性地通过实验识别这些调控元件。

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