Bio-products Laboratory, Central Leather Research Institute, Chennai, India.
Appl Biochem Biotechnol. 2010 Jan;160(1):30-9. doi: 10.1007/s12010-008-8452-4. Epub 2008 Dec 13.
A Bacillus subtilis (MTCC9102) isolate was shown to produce significant amount of keratinase under optimized conditions in solid-state fermentation using Horn meal as a substrate. Optimized value for moisture, inoculum, and aeration were found to be 100% (v/w), 50% (v/w), and 150% (w/w), respectively, and the optimum nitrogen source was peptone and carbon source was dextrose. Maximum keratinolytic activity was observed at 48 h after incubation, and the optimum age (24 h) of inoculum was significant. The influence of cultivation temperature and initial pH of the medium on keratinase production revealed the optimum values for the temperature and pH as 37 degrees C and 7, respectively. Maximum keratinase activity of the crude extract was 15,972 U/mg/ml. These results indicate that this bacterial strain shows a high biotechnological potential for keratinase production in solid-state fermentation, and use of the horn meal as the substrate can be implemented for keratinous solid wastes management.
枯草芽孢杆菌（MTCC9102）菌株在固态发酵中使用角蛋白作为底物，在优化条件下显示出大量产生角蛋白酶的能力。优化的水分、接种量和通气量分别为 100%（v/w）、50%（v/w）和 150%（w/w），最佳氮源为蛋白胨，最佳碳源为葡萄糖。培养 48 小时后观察到最大的角蛋白酶活性，接种的最佳龄（24 小时）是显著的。培养温度和培养基初始 pH 值对角蛋白酶生产的影响表明，温度和 pH 值的最佳值分别为 37°C 和 7。粗提物的最大角蛋白酶活性为 15972 U/mg/ml。这些结果表明，该细菌菌株在固态发酵中具有生产角蛋白酶的高生物技术潜力，并且可以对角蛋白固体废物管理使用角蛋白作为底物。
