Wang Xiao Suo, Stocker Roland
Centre for Vascular Research, Bosch Institute and Discipline of Pathology, The University of Sydney, Sydney, Australia.
Methods Mol Biol. 2008;477:49-63. doi: 10.1007/978-1-60327-517-0_5.
Atherosclerosis is associated with dysfunctional HDL, and oxidation of HDL is thought to give rise to HDL becoming dysfunctional. Lipoprotein oxidation represents a complex series of processes that can be assessed by various methods. In general, oxidation mediated by 1-electron or radical oxidants gives rise to lipid hydroperoxides (LOOHs) as the primary product. These LOOHs may then undergo further reactions giving rise to secondary lipid oxidation products and/or oxidation of lipoprotein-associated proteins. Thus, LOOHs specifically oxidize Met residues of apolipoprotein (apo) A-I and A-II (the major proteins of HDL) to MetO. Here we describe an HPLC-based method to detect oxidized HDL containing specifically oxidized forms of apoA-I and apoA-II. This method may be useful to assess the early stages of HDL oxidation in biological samples.
动脉粥样硬化与功能失调的高密度脂蛋白（HDL）相关，并且HDL的氧化被认为会导致HDL功能失调。脂蛋白氧化代表一系列复杂的过程，可通过多种方法进行评估。一般来说，由单电子或自由基氧化剂介导的氧化会产生脂质氢过氧化物（LOOHs）作为主要产物。这些LOOHs随后可能会发生进一步反应，产生二级脂质氧化产物和/或脂蛋白相关蛋白的氧化。因此，LOOHs会将载脂蛋白（apo）A-I和A-II（HDL的主要蛋白质）的甲硫氨酸（Met）残基特异性氧化为甲硫氨酸亚砜（MetO）。在此，我们描述一种基于高效液相色谱法（HPLC）的方法，用于检测含有apoA-I和apoA-II特定氧化形式的氧化型HDL。该方法可能有助于评估生物样品中HDL氧化的早期阶段。
