Yang Hongsong, Paromov Victor, Smith Milton, Stone William L
Department of Pediatrics, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN, USA.
Methods Mol Biol. 2008;477:277-92. doi: 10.1007/978-1-60327-517-0_21.
Antioxidant liposomes provide a unique means of delivering both water and/or lipid soluble antioxidants to tissues thereby affecting disease states or signal transduction pathways modulated by oxidative stress. Considerable evidence suggests that liposome-encapsulated antioxidants can be superior to the corresponding free antioxidants in this regard. This chapter will provide practical details on the preparation, characterization, and use of antioxidant liposomes. Methods will be described for the small-scale preparation (1 ml) and large-scale (100 ml/hour) preparation of antioxidant liposomes as well as the techniques for characterizing their size distribution and their physical and chemical stability. The use of antioxidant liposomes in an in vitro situation will also be detailed.
抗氧化脂质体提供了一种独特的方式，可将水溶性和/或脂溶性抗氧化剂输送到组织中，从而影响由氧化应激调节的疾病状态或信号转导途径。大量证据表明，在这方面，脂质体包裹的抗氧化剂可能优于相应的游离抗氧化剂。本章将提供有关抗氧化脂质体制备、表征和使用的实用细节。将描述小规模（1毫升）和大规模（每小时100毫升）制备抗氧化脂质体的方法，以及表征其尺寸分布及其物理和化学稳定性的技术。还将详细介绍抗氧化脂质体在体外情况下的使用。
