Laboratory of Nutrition and Cancer, Department of Physiology and Biophysics, Institute of Biology, State University of Campinas, UNICAMP, Campinas, Brazil.
Amino Acids. 2010 Jan;38(1):145-53. doi: 10.1007/s00726-008-0222-7. Epub 2008 Dec 13.
Branched-chain amino acids, especially leucine, exert regulatory influences on protein and carbohydrate metabolism, ribosome biogenesis and gene expression. This study investigated the effects of leucine in fibroblastic cells analysing viability, proliferation, morphology, proteolysis enzymes activities and protein turnover. After exposure to culture medium enriched with 25 or 50 microM leucine for 24, 48 and 72 h, Vero cells have no alterations on viability and morphology. Leucine-treated cells showed increase on alkaline phosphatase activity and proliferation. The protein synthesis was slightly increased, whereas the protein degradation showed a deep reduction after leucine incubation. The chymotrypsin-like, cathepsin B and H and calpain activities were decreased in leucine-treated cells. In conclusion, the proteolytic pathways and the total protein degradation were modulated by leucine in Vero cells. Our observations support the concept that Vero cells may represent a new model for protein turnover study.
支链氨基酸，特别是亮氨酸，对蛋白质和碳水化合物代谢、核糖体生物发生和基因表达具有调节作用。本研究分析了亮氨酸对成纤维细胞活力、增殖、形态、蛋白水解酶活性和蛋白质周转的影响。在暴露于富含 25 或 50 μM 亮氨酸的培养基中 24、48 和 72 小时后，Vero 细胞的活力和形态没有变化。亮氨酸处理的细胞表现出碱性磷酸酶活性和增殖增加。蛋白质合成略有增加，而亮氨酸孵育后蛋白质降解深度降低。亮氨酸处理的细胞中糜蛋白酶样、组织蛋白酶 B 和 H 和钙蛋白酶活性降低。总之，亮氨酸调节了 Vero 细胞中的蛋白水解途径和总蛋白降解。我们的观察结果支持 Vero 细胞可能代表蛋白质周转研究的新模式的概念。
