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[利用硅胶纯化产气荚膜梭菌和败血梭菌类毒素]

[Purification of the toxoids of Cl. Perfringens and Cl. pedematiens using silicagel].

作者信息

Efimova N P, Tul'bovich E V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1977(1):82-6.

PMID:190831
Abstract

A possibility was shown of purification of the concentrated toxoids of the Cl. perfringens and Cl. oedematiens species in examination of KSK-2, KSK-2,5; MCA-2 silicagels. The specific activity of the preparations obtained and the yield approached the analogous indices obtained in stratification of Sephadex G-75. The effect of preliminary silicagel treatment and also of the nature of buffer solutions on the efficacy of the process of purification of the gangrenous toxoids was established.

摘要

在对KSK - 2、KSK - 2.5和MCA - 2硅胶进行检测时,展示了一种纯化产气荚膜梭菌和水肿梭菌浓缩类毒素的可能性。所获得制剂的比活性和产量接近在Sephadex G - 75分层中获得的类似指标。确定了硅胶预处理以及缓冲溶液的性质对坏疽类毒素纯化过程效果的影响。

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