Gascan H, Lemetayer J
INSERM U211, Nantes, France.
Lymphokine Cytokine Res. 1991 Apr;10(1-2):115-8.
The control of leukemia inhibitory factor (LIF) secretion by interleukin-1 (IL-1) was studied in the HSB2 human T lymphoma cell line. We show that in the presence of phytohemagglutinin (PHA), the addition of IL-1 in the culture medium induced a strong increase in the level of secreted LIF, as detected with the DA1.a test cell line. Subclones of the HSB2 cell line obtained by limiting dilution, and selected for their high level of LIF secretion, after phorbol myristate acetate (PMA) treatment, were more carefully analyzed. In the same conditions of stimulation, a 50X increase in the amount of secreted LIF was observed with the 2B3 subclone, whereas the 3C2 subclone gave a very weak signal in response to the stimulation. Several other chemical reagents and a panel of recombinant cytokines including IL-2, -3, -4, -5, and -6, granulocyte monocyte colony-stimulating factor (GM-CSF), monocyte-CSF (M-CSF), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) were tested for their induction of LIF secretion. None of the other tested components was as potent as IL-1 (100 U/ml) and 1% PHA. A half maximal response was obtained with a concentration of 15-20 pM IL-1.
在HSB2人T淋巴瘤细胞系中研究了白细胞介素-1（IL-1）对白血病抑制因子（LIF）分泌的调控。我们发现，在存在植物血凝素（PHA）的情况下，向培养基中添加IL-1会导致分泌的LIF水平显著升高，这通过DA1.a测试细胞系检测到。对通过有限稀释获得的HSB2细胞系亚克隆进行了更仔细的分析，这些亚克隆经佛波醇肉豆蔻酸酯乙酸酯（PMA）处理后，因其高水平的LIF分泌而被挑选出来。在相同的刺激条件下，2B3亚克隆分泌的LIF量增加了50倍，而3C2亚克隆对刺激的反应信号非常微弱。还测试了其他几种化学试剂以及一组重组细胞因子，包括IL-2、-3、-4、-5和-6、粒细胞巨噬细胞集落刺激因子（GM-CSF）、单核细胞集落刺激因子（M-CSF）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）对LIF分泌的诱导作用。其他测试成分均不如IL-1（100 U/ml）和1%PHA有效。15-20 pM的IL-1浓度可产生半数最大反应。
