Parson Walther, Bandelt Hans-Jürgen
Institute of Legal Medicine, Innsbruck Medical University, Innsbruck, Austria.
Forensic Sci Int Genet. 2007 Mar;1(1):13-9. doi: 10.1016/j.fsigen.2006.11.003. Epub 2006 Dec 19.
Mitochondrial DNA analysis has become a vital niche in forensic science as it constitutes a powerful technique for low quality and low quantity DNA samples. For the forensic field it is important to employ standardized procedures based on scientific grounds, in order to have mtDNA evidence be accepted in court. Here, we modify and extend recommendations that were spelled out previously in the absence of solid knowledge about the worldwide phylogeny. Refinement of those earlier guidelines became necessary in regard to sample selection, amplification and sequencing strategies, as well as a posteriori quality control of mtDNA profiles. The notation of sequence data should thus reflect this growing knowledge.
线粒体DNA分析已成为法医学中的一个重要领域，因为它是处理低质量和低数量DNA样本的强大技术。对于法医学领域而言，基于科学依据采用标准化程序非常重要，以便线粒体DNA证据能在法庭上被接受。在此，我们修改并扩展了先前在缺乏关于全球系统发育的可靠知识的情况下制定的建议。鉴于样本选择、扩增和测序策略以及线粒体DNA图谱的事后质量控制，对这些早期指南进行完善变得必要。因此，序列数据的标注应反映这一日益增长的知识。
