Desmyter Stijn, Hoste Bernadette
National Institute for Criminalistics and Criminology, Vilvoordsesteenweg 100, B-1120 Brussels, Belgium.
Forensic Sci Int Genet. 2007 Jun;1(2):199-200. doi: 10.1016/j.fsigen.2007.02.002. Epub 2007 Mar 1.
The validation of an upgraded mtDNA profiling procedure served as a study on the electrophoresis-resequencing methods ABI377-LR5%-Sequence Navigator, ABI3100-POP4-SeqScape and ABI3100-POP6-SeqScape. The upgraded protocol was considered to be suitable for mitochondrial DNA sequencing of HV1 and HV2, with all three electrophoresis types tested. Nevertheless, the sequencing protocol had to be extended with additional primers to obtain a full double-stranded profile of samples with specific sequencing problems in the C-stretch of HV1 or HV2. The major differences between the tested electrophoresis types were observed in the detection limit and the automatic mixed base detection. But also the primer to sequencing start-distance depends on the method.
一种升级后的线粒体DNA（mtDNA）分析程序的验证，是针对电泳-重测序方法ABI377-LR5%-Sequence Navigator、ABI3100-POP4-SeqScape和ABI3100-POP6-SeqScape所开展的一项研究。升级后的方案被认为适用于HV1和HV2的线粒体DNA测序，且对所有三种电泳类型都进行了测试。然而，测序方案必须用额外的引物进行扩展，以获得在HV1或HV2的C-区段存在特定测序问题的样本的完整双链图谱。在所测试的电泳类型之间，主要差异体现在检测限和自动混合碱基检测方面。而且引物到测序起始距离也取决于方法。
