Anderung Cecilia, Persson Per, Bouwman Abigail, Elburg Rengert, Götherström Anders
Department of Evolution, Evolutionary Biology Centre, Uppsala Univeristy, Norbyvägen, Uppsala, Sweden.
Forensic Sci Int Genet. 2008 Mar;2(2):104-7. doi: 10.1016/j.fsigen.2007.09.004. Epub 2007 Nov 19.
The major problems concerning ancient DNA studies are related to the amount of extractable DNA and the precautions needed to avoid contamination. From the very first step of the analyses, the DNA extraction, these problems must be confronted. There are several extraction methods available for DNA in ancient tissue; several of them are complicated and time consuming, and none of the methods have reached an acceptance level such that they are routinely used on a widespread basis. Here we investigate the efficiency of two methods, one based on magnetic separation of the targeted molecules, and one based on silica binding. The efficiency rate of these two on the material studied seems to be identical. The silica binding method has the benefit of relative simplicity, but the magnetic separation technique also has advantages. For example, it is possible to reuse the extract several times for different loci, and it is possible to concentrate all extracted DNA from one locus into one PCR.
古代DNA研究的主要问题与可提取DNA的量以及避免污染所需的预防措施有关。从分析的第一步，即DNA提取开始，就必须面对这些问题。古代组织中的DNA有几种提取方法；其中一些方法复杂且耗时，而且没有一种方法达到被广泛常规使用的接受程度。在这里，我们研究了两种方法的效率，一种基于靶向分子的磁分离，另一种基于硅胶结合。这两种方法在所研究材料上的效率似乎相同。硅胶结合法的优点是相对简单，但磁分离技术也有优势。例如，可以将提取物多次用于不同的基因座，并且可以将来自一个基因座的所有提取DNA浓缩到一个聚合酶链反应（PCR）中。
