2006年GEP-ISFG线粒体DNA协作研究：关于解读、伪像及DNA混合物的思考
2006 GEP-ISFG collaborative exercise on mtDNA: reflections about interpretation, artefacts, and DNA mixtures.
作者信息
Prieto L, Alonso A, Alves C, Crespillo M, Montesino M, Picornell A, Brehm A, Ramírez J L, Whittle M R, Anjos M J, Boschi I, Buj J, Cerezo M, Cardoso S, Cicarelli R, Comas D, Corach D, Doutremepuich C, Espinheira R M, Fernández-Fernández I, Filippini S, Garcia-Hirschfeld Julia, González A, Heinrichs B, Hernández A, Leite F P N, Lizarazo R P, López-Parra A M, López-Soto M, Lorente J A, Mechoso B, Navarro I, Pagano S, Pestano J J, Puente J, Raimondi E, Rodríguez-Quesada A, Terra-Pinheiro M F, Vidal-Rioja L, Vullo C, Salas A
机构信息
Comisaría General de Policía Científica, DNA Laboratory, Madrid, Spain.
出版信息
Forensic Sci Int Genet. 2008 Mar;2(2):126-33. doi: 10.1016/j.fsigen.2007.10.010. Epub 2007 Nov 9.
We report the results of the seventh edition of the GEP-ISFG mitochondrial DNA (mtDNA) collaborative exercise. The samples submitted to the participant laboratories were blood stains from a maternity case and simulated forensic samples, including a case of mixture. The success rate for the blood stains was moderate ( approximately 77%); even though four inexperienced laboratories concentrated about one-third of the total errors. A similar success was obtained for the analysis of mixed samples (78.8% for a hair-saliva mixture and 69.2% for a saliva-saliva mixture). Two laboratories also dissected the haplotypes contributing to the saliva-saliva mixture. Most of the errors were due to reading problems and misinterpretation of electropherograms, demonstrating once more that the lack of a solid devised experimental approach is the main cause of error in mtDNA testing.
我们报告了第七届GEP-ISFG线粒体DNA（mtDNA）协作实验的结果。提交给参与实验室的样本包括一个产妇案例的血迹以及模拟法医样本，其中有一个混合样本案例。血迹样本的成功率适中（约77%）；尽管四个缺乏经验的实验室集中了约三分之一的总误差。混合样本分析也取得了类似的成功率（毛发-唾液混合样本为78.8%，唾液-唾液混合样本为69.2%）。两个实验室还剖析了构成唾液-唾液混合样本的单倍型。大多数误差是由于阅读问题和电泳图解读错误造成的，这再次表明缺乏可靠的实验设计方法是mtDNA检测中误差的主要原因。
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