French D J, Howard R L, Gale N, Brown T, McDowell D G, Debenham P G
Innovation and Support Team, LGC, Middlesex, UK.
Forensic Sci Int Genet. 2008 Sep;2(4):333-9. doi: 10.1016/j.fsigen.2008.04.007. Epub 2008 Jun 10.
Current forensic DNA profiling methods rely on the analysis of samples at specialised laboratories with an average turnaround time of several days. The ability to rapidly determine a partial profile of short tandem repeats at the point-of-arrest would be of great benefit to police forces around the world, for example enabling a suspect to be rapidly included or excluded from an investigation. We have developed a homogeneous PCR method for the interrogation of STR loci utilising fluorescent oligonucleotide probes and melting curve analysis. Alleles of the D18S51, TH01 and D8S1179 loci were differentiated and identified on the basis of target length and probe melting temperature. Assay performance was evaluated by comparing melting peak data with the AmpFlSTR SGM Plus system. The method is compatible with direct analysis of unpurified buccal swab samples, enabling a partial STR profile to be generated within 1h.
当前的法医DNA分型方法依赖于在专门实验室对样本进行分析，平均周转时间为几天。在逮捕现场快速确定短串联重复序列的部分图谱的能力，将对世界各地的警察部队大有裨益，例如能够迅速将嫌疑人纳入或排除在调查之外。我们开发了一种利用荧光寡核苷酸探针和熔解曲线分析来检测STR基因座的均相PCR方法。根据目标长度和探针熔解温度对D18S51、TH01和D8S1179基因座的等位基因进行区分和鉴定。通过将熔解峰数据与AmpFlSTR SGM Plus系统进行比较来评估检测性能。该方法与未经纯化的口腔拭子样本的直接分析兼容，能够在1小时内生成部分STR图谱。
