Ooe Norihisa, Kobayashi Kentaro, Motonaga Kozo, Saito Koichi, Kaneko Hideo
Environmental Health Science Laboratory, Sumitomo Chemical Co, Ltd, Osaka, Japan.
Biochem Biophys Res Commun. 2009 Jan 23;378(4):761-5. doi: 10.1016/j.bbrc.2008.11.137. Epub 2008 Dec 10.
While neurotrophin is known to be involved in a variety of neuronal functions inducing several immediate early genes and activating several signaling molecules, the correspondence with downstream cascades remains to be defined in detail. Here we show that a bHLH-PAS transcription factor, NXF, is a new member genes under the control of neurotrophin. The PI3K-Akt system, an important cell-protection-signaling cascade under the control of the neurotrophin receptor, was also revealed to contribute to the mechanism of NXF mRNA induction. Activation of MAPK under the control of the neurotrophin receptor resulted in NXF protein phosphorylation as well as enhancement of NXF transcriptional activity. This newly identified NXF gene system may provide a new insight into neurotrophin biology, which reflects the target gene functions.
虽然已知神经营养因子参与多种神经元功能，可诱导多个立即早期基因并激活多种信号分子，但其与下游级联反应的对应关系仍有待详细确定。在此我们表明，一种bHLH-PAS转录因子NXF是受神经营养因子控制的新成员基因。PI3K-Akt系统是在神经营养因子受体控制下的重要细胞保护信号级联反应，也被发现参与了NXF mRNA诱导机制。神经营养因子受体控制下的MAPK激活导致NXF蛋白磷酸化以及NXF转录活性增强。这个新鉴定的NXF基因系统可能为神经营养因子生物学提供新的见解，这反映了靶基因的功能。
