Bueno Regina, Carvalho Neta Alcina V, Xavier Mariana N, Oliveira Raquel G, Diniz Soraia A, Melo Maria N, Santos Renato L
Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Vet Immunol Immunopathol. 2009 Feb 15;127(3-4):332-9. doi: 10.1016/j.vetimm.2008.10.335. Epub 2008 Nov 7.
Nramp1 (Slc11a1) is linked to resistance to Leishmania in mice, but its role in canine leishmaniasis is not clear. In this study we sequenced the Nramp1 cDNA from dogs whose macrophages allowed or restricted intracellular growth of Leishmania chagasi. Peripheral blood monocyte-derived macrophages were isolated from 29 dogs, cultured and inoculated with L. chagasi. This approach resulted in the identification of dogs whose macrophages were resistant or susceptible to L. chagasi. Nramp1 cDNA sequences of these dogs were identical. mRNA levels of Nramp1, IFNgamma, IL-4 and the subunit p35 of IL-12 were assessed in the spleen of naturally infected symptomatic and asymptomatic dogs in comparison to uninfected controls. Although not statistically significant, asymptomatic dogs had a tendency for higher levels of Nramp1 mRNA (p = 0.11). Expression of Nramp1 was then compared between phenotypically resistant and susceptible dogs, without any significant difference between these groups.
Nramp1（Slc11a1）与小鼠对利什曼原虫的抗性有关，但其在犬利什曼病中的作用尚不清楚。在本研究中，我们对犬的Nramp1 cDNA进行了测序，这些犬的巨噬细胞允许或限制恰加斯利什曼原虫在细胞内生长。从29只犬中分离出外周血单核细胞来源的巨噬细胞，进行培养并接种恰加斯利什曼原虫。通过这种方法鉴定出了巨噬细胞对恰加斯利什曼原虫具有抗性或易感性的犬。这些犬的Nramp1 cDNA序列相同。与未感染的对照相比，评估了自然感染的有症状和无症状犬脾脏中Nramp1、IFNγ、IL-4和IL-12亚基p35的mRNA水平。尽管无统计学意义，但无症状犬的Nramp1 mRNA水平有升高趋势（p = 0.11）。然后比较了表型抗性和易感犬之间Nramp1的表达，两组之间无显著差异。
