The Boron-doped carbon nanotubes (BCNTs) modified glassy carbon (GC) electrode was obtained simply and used for highly selective and sensitive determination of dopamine (DA). Comparing with the bare GC and CNTs/GC electrodes, the BCNTs have higher catalytic activity toward the oxidation of DA and ascorbic acid (AA). Moreover, the voltammetric peaks of AA and DA were separated enough (ca. 238 mV) at the BCNTs/GC electrode, which is superior to that at the CNTs/GC electrode (ca. 122 mV). Thus, the selective determination of DA was carried out successfully in the presence of AA. A wide concentration range (2.0 x 10(-8)-7.5 x 10(-5)M) and low detection limit (1.4 nM, S/N=3) for the DA detection were obtained. The possibility of the BCNTs/GC electrode for the determination of DA in human blood serum has also been evaluated. The advantageous properties of this electrode for the DA determination lie in its excellent catalytic activity, selectivity and simplicity. The more edge plane sites presented on the BCNTs surface were partially responsible for its good analytical behavior.