Neupert Susanne, Russell William K, Russell David H, López Juan D, Predel Reinhard, Nachman Ronald J
Areawide Pest Management Research, Southern Plains Agricultural Research Center, USDA, 2881 F&B Road, College Station, TX 77845, USA.
Peptides. 2009 Mar;30(3):483-8. doi: 10.1016/j.peptides.2008.11.009. Epub 2008 Nov 25.
Recently, the peptidomic analysis of neuropeptides from the retrocerebral complex and abdominal perisympathetic organs of polyphagous stinkbugs (Pentatomidae) revealed the group-specific sequences of pyrokinins, CAPA peptides (CAPA-periviscerokinins/PVKs and CAPA-pyrokinin), myosuppressin, corazonin, adipokinetic hormone, and short neuropeptide F. In this study, we used mass spectrometric profiling of nervous tissue from the species-rich taxon Hemiptera to identify products of two previously unobserved neuropeptide genes from these species, namely allatotropin-related peptide (ATRP) and tachykinin-related peptides (TKRPs). Since neither TKRPs nor allatotropin are accumulated in neurohemal organs, immunocytochemical data were analyzed to find potential accumulation sites within the central nervous system. By mass spectrometry, TKRPs were found to be accumulated in the antennal lobes, and ATRP was identified in the most posterior region of the abdominal ventral nerve cord and fourth abdominal nerves. In addition to neuropeptides from stink bugs, TKRPs and ATRP were also identified from the distantly related bugs Oncopeltus fasciatus (Lygaeidae) and Pyrrhocoris apterus (Pyrrhocoridae). In total, six TKRPs and one ATRP from each species could be elucidated by tandem mass spectrometry. The ATRP of all species is sequence-identical with Locusta migratoria accessory gland myotropin-1 (Lom-AG-MT-1), a member of the highly conserved insect allatotropin family.
最近，对多食性椿象（蝽科）后脑复合体和腹部交感神经器官中的神经肽进行的肽组学分析揭示了速激肽、CAPA肽（CAPA-内脏周围激肽/PVKs和CAPA-速激肽）、肌抑制素、促心搏素、脂肪动激素和短神经肽F的类群特异性序列。在本研究中，我们利用对物种丰富的半翅目类群神经组织进行质谱分析，来鉴定这些物种中两个先前未观察到的神经肽基因的产物，即促咽侧体素相关肽（ATRP）和速激肽相关肽（TKRPs）。由于TKRPs和促咽侧体素均未在神经血器官中积累，因此分析了免疫细胞化学数据以寻找中枢神经系统内的潜在积累位点。通过质谱分析，发现TKRPs在触角叶中积累，而ATRP在腹侧神经索的最后部区域和第四腹神经中被鉴定出来。除了椿象的神经肽外，还从远缘的臭虫烟草盲蝽（长蝽科）和红蝽（红蝽科）中鉴定出了TKRPs和ATRP。通过串联质谱总共可以阐明每个物种的六种TKRPs和一种ATRP。所有物种的ATRP与飞蝗副腺肌动蛋白-1（Lom-AG-MT-1）序列相同，后者是高度保守的昆虫促咽侧体素家族的成员。
