• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Mass spectral imaging and profiling of neuropeptides at the organ and cellular domains.对神经肽进行器官和细胞水平的质谱成像和分析。
Anal Bioanal Chem. 2010 Aug;397(8):3185-93. doi: 10.1007/s00216-010-3723-7. Epub 2010 Apr 25.
2
High Throughput In Situ DDA Analysis of Neuropeptides by Coupling Novel Multiplex Mass Spectrometric Imaging (MSI) with Gas-Phase Fractionation.通过将新型多重质谱成像（MSI）与气相分级分离相结合对神经肽进行高通量原位DDA分析。
J Am Soc Mass Spectrom. 2015 Dec;26(12):1992-2001. doi: 10.1007/s13361-015-1265-0. Epub 2015 Oct 5.
3
Mapping of neuropeptides in the crustacean stomatogastric nervous system by imaging mass spectrometry.通过成像质谱法对甲壳类动物口胃神经系统中的神经肽进行定位。
J Am Soc Mass Spectrom. 2013 Jan;24(1):134-47. doi: 10.1007/s13361-012-0502-z. Epub 2012 Nov 29.
4
Single-cell MALDI: a new tool for direct peptide profiling.单细胞基质辅助激光解吸电离：一种用于直接肽谱分析的新工具。
Trends Biotechnol. 2000 Apr;18(4):151-60. doi: 10.1016/s0167-7799(00)01427-x.
5
A rapid MALDI-TOF mass spectrometry workflow for Drosophila melanogaster differential neuropeptidomics.一种用于黑腹果蝇差异神经肽组学的快速 MALDI-TOF 质谱工作流程。
Mol Brain. 2013 Dec 27;6:60. doi: 10.1186/1756-6606-6-60.
6
In situ identification and mapping of neuropeptides from the stomatogastric nervous system of Cancer borealis.原位鉴定和绘制来自北方黄道蟹口胃神经系统的神经肽图谱。
Rapid Commun Mass Spectrom. 2014 Nov 30;28(22):2437-44. doi: 10.1002/rcm.7037.
7
Mass spectrometric map of neuropeptide expression in Ascaris suum.猪蛔虫神经肽表达的质谱图。
J Comp Neurol. 2005 Aug 8;488(4):396-413. doi: 10.1002/cne.20587.
8
Heterogeneity within MALDI samples as revealed by mass spectrometric imaging.质谱成像揭示的基质辅助激光解吸电离（MALDI）样本中的异质性。
Anal Chem. 2000 Jan 1;72(1):30-6. doi: 10.1021/ac9908997.
9
Three dimensional mapping of neuropeptides and lipids in crustacean brain by mass spectral imaging.通过质谱成像对甲壳类动物大脑中的神经肽和脂质进行三维映射。
J Am Soc Mass Spectrom. 2009 Jun;20(6):1068-77. doi: 10.1016/j.jasms.2009.01.017. Epub 2009 Jan 31.
10
MALDI imaging mass spectrometry of neuropeptides in Parkinson's disease.帕金森病中神经肽的基质辅助激光解吸电离成像质谱分析
J Vis Exp. 2012 Feb 14(60):3445. doi: 10.3791/3445.

引用本文的文献

1
Mass Spectrometry Imaging.质谱成像
Mass Spectrom (Tokyo). 2022;11(1):A0102. doi: 10.5702/massspectrometry.A0102. Epub 2022 Feb 25.
2
Recent advances in mass spectrometry analysis of neuropeptides.近年来神经肽的质谱分析进展。
Mass Spectrom Rev. 2023 Mar;42(2):706-750. doi: 10.1002/mas.21734. Epub 2021 Sep 24.
3
Neuropeptide Localization in : From the Central Nervous System to Subcellular Compartments.神经肽在：从中枢神经系统到亚细胞区室的定位
Front Mol Neurosci. 2021 May 20;14:670303. doi: 10.3389/fnmol.2021.670303. eCollection 2021.
4
MALDI Mass Spectrometry Imaging of Peptides in Medicago truncatula Root Nodules.毛苕子根瘤中肽的 MALDI 质谱成像分析。
Methods Mol Biol. 2020;2139:341-351. doi: 10.1007/978-1-0716-0528-8_25.
5
Comparison of Vacuum MALDI and AP-MALDI Platforms for the Mass Spectrometry Imaging of Metabolites Involved in Salt Stress in .真空基质辅助激光解吸电离和常压基质辅助激光解吸电离平台用于质谱成像分析参与盐胁迫的代谢物的比较
Front Plant Sci. 2018 Aug 28;9:1238. doi: 10.3389/fpls.2018.01238. eCollection 2018.
6
Effective Sample Preparations in Imaging Mass Spectrometry.成像质谱中的有效样品制备
Mass Spectrom (Tokyo). 2014;3(Spec Issue):S0029. doi: 10.5702/massspectrometry.S0029. Epub 2014 May 1.
7
Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples.基于质谱法对小体积样本中内源性肽和代谢物的表征。
Biochim Biophys Acta. 2015 Jul;1854(7):732-40. doi: 10.1016/j.bbapap.2015.01.008. Epub 2015 Jan 21.
8
Mass spectrometric analysis of spatio-temporal dynamics of crustacean neuropeptides.甲壳类动物神经肽时空动态的质谱分析
Biochim Biophys Acta. 2015 Jul;1854(7):798-811. doi: 10.1016/j.bbapap.2014.10.023. Epub 2014 Nov 4.
9
In situ identification and mapping of neuropeptides from the stomatogastric nervous system of Cancer borealis.原位鉴定和绘制来自北方黄道蟹口胃神经系统的神经肽图谱。
Rapid Commun Mass Spectrom. 2014 Nov 30;28(22):2437-44. doi: 10.1002/rcm.7037.
10
Quantitative neuropeptidomics study of the effects of temperature change in the crab Cancer borealis.北方黄道蟹温度变化影响的定量神经肽组学研究
J Proteome Res. 2014 Dec 5;13(12):5767-76. doi: 10.1021/pr500742q. Epub 2014 Sep 29.

本文引用的文献

1
On-tissue N-terminal peptide derivatizations for enhancing protein identification in MALDI mass spectrometric imaging strategies.组织内 N 端肽衍生化增强 MALDI 质谱成像策略中的蛋白质鉴定。
Anal Chem. 2009 Oct 15;81(20):8305-17. doi: 10.1021/ac901043n.
2
Characterization of the Carcinus maenas neuropeptidome by mass spectrometry and functional genomics.通过质谱和功能基因组学对绿黄道蟹神经肽组进行表征。
Gen Comp Endocrinol. 2009 May;161(3):320-34. doi: 10.1016/j.ygcen.2009.01.015. Epub 2009 Jan 31.
3
Three dimensional mapping of neuropeptides and lipids in crustacean brain by mass spectral imaging.通过质谱成像对甲壳类动物大脑中的神经肽和脂质进行三维映射。
J Am Soc Mass Spectrom. 2009 Jun;20(6):1068-77. doi: 10.1016/j.jasms.2009.01.017. Epub 2009 Jan 31.
4
Neuropeptides in Heteroptera: identification of allatotropin-related peptide and tachykinin-related peptides using MALDI-TOF mass spectrometry.异翅亚目昆虫中的神经肽：使用基质辅助激光解吸电离飞行时间质谱法鉴定促咽侧体素相关肽和速激肽相关肽
Peptides. 2009 Mar;30(3):483-8. doi: 10.1016/j.peptides.2008.11.009. Epub 2008 Nov 25.
5
Allatotropin-related peptide in cockroaches: identification via mass spectrometric analysis of single identified neurons.蟑螂中与促咽侧体素相关的肽：通过对单个已鉴定神经元的质谱分析进行鉴定
Peptides. 2009 Mar;30(3):489-94. doi: 10.1016/j.peptides.2008.10.023. Epub 2008 Nov 24.
6
Combining bottom-up and top-down mass spectrometric strategies for de novo sequencing of the crustacean hyperglycemic hormone from Cancer borealis.结合自下而上和自上而下的质谱策略对北方黄道蟹的甲壳类高血糖激素进行从头测序。
Anal Chem. 2009 Jan 1;81(1):240-7. doi: 10.1021/ac801910g.
7
Neuropeptides associated with the regulation of feeding in insects.与昆虫进食调节相关的神经肽。
Gen Comp Endocrinol. 2009 May 15;162(1):93-104. doi: 10.1016/j.ygcen.2008.08.003. Epub 2008 Aug 19.
8
Molecular evolution of neuropeptides in the genus Drosophila.果蝇属神经肽的分子进化
Genome Biol. 2008;9(8):R131. doi: 10.1186/gb-2008-9-8-r131. Epub 2008 Aug 21.
9
SIMS and MALDI MS imaging of the spinal cord.脊髓的二次离子质谱成像（SIMS）和基质辅助激光解吸/电离质谱成像（MALDI MS）
Proteomics. 2008 Sep;8(18):3746-54. doi: 10.1002/pmic.200800127.
10
Quantitative measurements of cell-cell signaling peptides with single-cell MALDI MS.利用单细胞基质辅助激光解吸电离质谱对细胞间信号肽进行定量测量。
Anal Chem. 2008 Sep 15;80(18):7128-36. doi: 10.1021/ac8010389. Epub 2008 Aug 16.

对神经肽进行器官和细胞水平的质谱成像和分析。

Mass spectral imaging and profiling of neuropeptides at the organ and cellular domains.

机构信息

Department of Chemistry & School of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.

出版信息

Anal Bioanal Chem. 2010 Aug;397(8):3185-93. doi: 10.1007/s00216-010-3723-7. Epub 2010 Apr 25.

DOI:10.1007/s00216-010-3723-7
PMID:20419488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6314848/
Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical method that is well suited for determining molecular weights of peptides and proteins from complex samples. MALDI-MS can be used to profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation. Furthermore, the recently developed MALDI imaging technique enables mapping of the spatial distribution of signaling molecules in tissue samples. Several examples of signaling molecule analysis at the single-cell and single-organ levels using MALDI-MS technology are highlighted followed by an outlook of future directions.

摘要

基质辅助激光解吸/电离（MALDI）质谱（MS）是一种快速、灵敏的分析方法，非常适合从复杂样品中测定肽和蛋白质的分子量。MALDI-MS 可用于分析单细胞和小组织样品中的肽和蛋白质，而无需进行广泛的样品制备。此外，最近开发的 MALDI 成像技术能够在组织样品中绘制信号分子的空间分布图谱。本文突出介绍了使用 MALDI-MS 技术在单细胞和单器官水平上分析信号分子的几个实例，然后展望了未来的方向。