McCue B A, Park Y H, Brazzell R K, Boltralik J J
Research and Development, Alcon Laboratories, Inc., Fort Worth, TX 76134.
J Chromatogr. 1991 Apr 19;565(1-2):255-64. doi: 10.1016/0378-4347(91)80388-s.
A sensitive and selective gas chromatographic-electron-capture assay was developed for the determination of the aldose reductase inhibitor imirestat in lens and plasma. The method involves solid-phase extraction of drug and internal standard from the plasma specimen or lens sample homogenate using "Baker"-10 SPE extraction columns followed by derivatization with pentafluorobenzyl bromide and further purification. Derivatives of drug and internal standard were separated on a fused-silica capillary column and analyzed using a 63Ni electron-capture detector. The limit of detection was 2.5 ng per lens or ml of plasma. The method was used to evaluate the pharmacokinetics of imirestat in human subjects and to quantitate imirestat in animal lens tissue following topical ocular administration.
开发了一种灵敏且具选择性的气相色谱 - 电子捕获分析法，用于测定晶状体和血浆中的醛糖还原酶抑制剂依米司他。该方法包括使用“贝克” - 10固相萃取柱从血浆标本或晶状体样品匀浆中固相萃取药物和内标，随后用五氟苄基溴进行衍生化并进一步纯化。药物和内标的衍生物在熔融石英毛细管柱上分离，并用63Ni电子捕获检测器进行分析。检测限为每个晶状体或每毫升血浆2.5纳克。该方法用于评估依米司他在人体受试者中的药代动力学，并在局部眼部给药后对动物晶状体组织中的依米司他进行定量。
