白藜芦醇可减轻大鼠肝脏缺血/再灌注诱导的氧化应激和组织学改变。
Resveratrol attenuates oxidative stress and histological alterations induced by liver ischemia/reperfusion in rats.
作者信息
Gedik Ercan, Girgin Sadullah, Ozturk Hayrettin, Obay Basra-Deniz, Ozturk Hulya, Buyukbayram Huseyin
机构信息
Department of General Surgery, Medical School, Dicle University, Diyarbakir 21280, Turkey.
出版信息
World J Gastroenterol. 2008 Dec 14;14(46):7101-6. doi: 10.3748/wjg.14.7101.
AIM
To investigate the effects of resveratrol on liver ischemia/reperfusion (I/R) injury in rats.
METHODS
A total of 40 male Sprague-Dawley rats weighing 240-290 g were randomized into four groups of ten: (1) controls: data from unmanipulated animals; (2) sham group: rats subjected to the surgical procedure, except for liver I/R, and given saline; (3) I/R group: rats underwent liver ischemia for 45 min followed by reperfusion for 45 min; (4) I-R/Resveratrol group: rats pretreated with resveratrol (10 micromol/L, iv). Liver tissues were obtained to determine antioxidant enzyme levels and for biochemical and histological evaluation.
RESULTS
Plasma aminotransferase activities were higher in the I/R group than in the I-R/Resveratrol group. Malondialdehyde levels and the hepatic injury score decreased, while superoxide dismutase, catalase, and glutathione peroxidase levels increased in group 4 compared to group 3. In group 4, histopathological changes were significantly attenuated in resveratrol-treated livers.
CONCLUSION
These results suggest that resveratrol has protective effects against hepatic I/R injury, and is a potential therapeutic drug for ischemia reperfusion-related liver injury.
目的
探讨白藜芦醇对大鼠肝脏缺血/再灌注（I/R）损伤的影响。
方法
将40只体重240 - 290克的雄性Sprague-Dawley大鼠随机分为四组，每组10只：（1）对照组：未进行处理动物的数据；（2）假手术组：接受手术操作，但未进行肝脏I/R，给予生理盐水；（3）I/R组：大鼠肝脏缺血45分钟，随后再灌注45分钟；（4）I-R/白藜芦醇组：用白藜芦醇（10微摩尔/升，静脉注射）预处理的大鼠。获取肝脏组织以测定抗氧化酶水平，并进行生化和组织学评估。
结果
I/R组血浆转氨酶活性高于I-R/白藜芦醇组。与第3组相比，第4组丙二醛水平和肝损伤评分降低，而超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶水平升高。在第4组中，白藜芦醇处理的肝脏组织病理学变化明显减轻。
结论
这些结果表明白藜芦醇对肝脏I/R损伤具有保护作用，是一种治疗缺血再灌注相关肝损伤的潜在药物。
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