Kim Su Kang, Kang Sang Wook, Jin Seul A, Ban Ju Yeon, Hong Seoung-Jin, Park Min-Su
Department of Biomedical Laboratory Science, Catholic Kwandong University, Gangneung, Republic of Korea.
Department of Dental Pharmacology, College of Dentistry, Dankook University, Cheonan, Republic of Korea.
Transplant Proc. 2019 Oct;51(8):2823-2827. doi: 10.1016/j.transproceed.2019.02.056. Epub 2019 Sep 4.
Hepatic ischemia reperfusion (I/R) injury is regarded as a serious concern in clinical practice. Citric acid reduces oxidative stress and inflammation during hypoxia and reoxygenation. Our objective was to investigate the protective effect of citric acid against hepatic I/R injury in rats.
We fed Sprague-Dawley rats either citric acid (100 mg/kg/d) or saline. One week later, ischemia was induced by clamping the rats' common hepatic artery and portal vein for 30 minutes. The rats were randomly divided into 3 major groups that were treated as follows: 1. the sham operated group; 2. the I/R group; and 3. the I/R-citric acid group.
Compared to the sham group, the I/R group had higher expression of aspartate aminotransferase and alanine aminotransferase and lower expression of catalase, superoxide dismutase, glutathione peroxidase, antioxidant, nitric oxide, and albumin. Compared to the I/R group, the I/R-citric acid group had higher expression of catalase, superoxide dismutase, antioxidants, and nitric oxide, and lower expression of aspartate aminotransferase and alanine aminotransferase.
These results suggest that citric acid therapy has significant therapeutic potential in ischemic liver injury.
肝缺血再灌注（I/R）损伤是临床实践中备受关注的严重问题。柠檬酸可减轻缺氧和复氧过程中的氧化应激及炎症反应。我们的目的是研究柠檬酸对大鼠肝缺血再灌注损伤的保护作用。
我们给斯普拉格-道利大鼠喂食柠檬酸（100mg/kg/d）或生理盐水。一周后，通过夹闭大鼠的肝总动脉和门静脉30分钟诱导缺血。大鼠被随机分为3个主要组，治疗方式如下：1. 假手术组；2. 缺血再灌注组；3. 缺血再灌注-柠檬酸组。
与假手术组相比，缺血再灌注组天冬氨酸转氨酶和丙氨酸转氨酶的表达较高，而过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、抗氧化剂、一氧化氮和白蛋白的表达较低。与缺血再灌注组相比，缺血再灌注-柠檬酸组过氧化氢酶、超氧化物歧化酶、抗氧化剂和一氧化氮的表达较高，天冬氨酸转氨酶和丙氨酸转氨酶的表达较低。
这些结果表明柠檬酸疗法在缺血性肝损伤中具有显著的治疗潜力。
