Liu Bing, Zhang Hong, Luo Xiaohong, Xie Yi, Hao Jifang, Zhou Qingming, Duan Xin, Wang Yanling, Zhao Weiping
Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., 730000, Lanzhou, China.
J Cancer Res Clin Oncol. 2009 Jul;135(7):925-32. doi: 10.1007/s00432-008-0528-6. Epub 2008 Dec 16.
The aim of this study is to evaluate the effect of carbon-beam irradiation on adenovirus-mediated p53 transfer in human cervix adenocarcinoma.
The HeLa cells pre-exposed to carbon-beam or gamma-ray, were infected with replication-deficient adenovirus recombinant vectors, containing human wild-type p53 (AdCMV-p53) and green fluorescent protein (GFP) (AdCMV-GFP), respectively. The GFP transfer and p53 expression were detected by flow cytometric analysis.
The GFP transfer frequency in C-beam with AdCMV-GFP groups was 38-50% more than that in gamma-ray with AdCMV-GFP groups. The percentage of p53 positive cells in the C-beam with AdCMV-p53 groups was 34-55.6% more than that in gamma-ray with AdCMV-p53 groups (p < 0.05), suggesting that subclinical-dose C-beam irradiation could significantly promote exogenous p53 transfer and p53 expression, and extend the duration of p53 expression in the HeLa cells. The expression of p21 increased with p53 expression in HeLa cells. The survival fractions for the 0.5-1.0 Gy C-beam with AdCMV-p53 groups were 38-43% less than those for the isodose gamma-ray with AdCMV-p53 groups, and 31-40% less than those for the C-beam only groups (p < 0.05).
The subclinical-dose C-beam irradiation could significantly promote the transfer and expression of exogenous p53, extend the duration of p53 expression, and enhance the suppression of p53 on cervix adenocarcinoma cells.
本研究旨在评估碳离子束辐照对人宫颈腺癌中腺病毒介导的p53基因转移的影响。
将预先暴露于碳离子束或γ射线的HeLa细胞分别用携带人野生型p53（AdCMV-p53）和绿色荧光蛋白（GFP）（AdCMV-GFP）的复制缺陷型腺病毒重组载体进行感染。通过流式细胞术分析检测GFP转移和p53表达情况。
AdCMV-GFP组中，碳离子束照射组的GFP转移频率比γ射线照射组高38%-50%。AdCMV-p53组中，碳离子束照射组的p53阳性细胞百分比比γ射线照射组高34%-55.6%（p<0.05），这表明亚临床剂量的碳离子束辐照可显著促进外源性p53转移和p53表达，并延长HeLa细胞中p53表达的持续时间。HeLa细胞中p21的表达随p53表达增加而增加。AdCMV-p53组中，0.5-1.0 Gy碳离子束照射后的存活分数比等剂量γ射线照射组低38%-43%，比仅碳离子束照射组低31%-40%（p<0.05）。
亚临床剂量的碳离子束辐照可显著促进外源性p53的转移和表达，延长p53表达的持续时间，并增强p53对宫颈腺癌细胞的抑制作用。
