低剂量碳离子辐射诱导AdCMV-p53在人肝癌细胞中的高效转移与表达

High-efficiency transfer and expression of AdCMV-p53 in human hepatocellular carcinoma cells induced by low-dose carbon-ion radiation.

作者信息

Liu Bing, Zhang Hong, Luo Xiaohong, Xie Yi, Hao Jifang, Duan Xin, Zhou Qingming

机构信息

Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.

出版信息

Eur J Gastroenterol Hepatol. 2008 Sep;20(9):860-4. doi: 10.1097/MEG.0b013e3282f945e8.

DOI:10.1097/MEG.0b013e3282f945e8

PMID:18794599

Abstract

OBJECTIVE

To investigate whether the irradiation with C-beam could enhance adenovirus-mediated transfer and expression of p53 in human hepatocellular carcinoma.

MATERIALS AND METHODS

HepG2 cells were exposed to C-beam or gamma-ray and then infected with replication-deficient adenovirus recombinant vectors containing human wild-type p53 or green fluorescent protein, respectively. The transfer efficiency and expression level of the exogenous gene were detected by flow cytometric analysis. Cell survival fraction was detected by clonogenic assay.

RESULTS

The transfer frequency in C-beam or gamma-irradiated groups increased by 50-83% and 5.7-38.0% compared with the control, respectively (P<0.05). Compared with C-beam alone, p53 alone, and gamma-ray with p53, the percentages of p53 positive cells for 1 Gy C-beam with p53 increased by 56.0-72.0%, 63.5-82.0%, and 31.3-72.5% on first and third day after the treatments, respectively (P<0.05). The survival fractions for the 2 Gy C-beam and AdCMV-p53 infection groups decreased to approximately 2%.

CONCLUSION

C-beam irradiation could significantly promote AdCMV-green fluorescent protein transfer and expression of p53.

摘要

目的

研究碳离子束照射是否能增强腺病毒介导的p53基因在人肝癌细胞中的转导与表达。

材料与方法

将HepG2细胞分别用碳离子束或γ射线照射，然后分别感染含人野生型p53或绿色荧光蛋白的复制缺陷型腺病毒重组载体。采用流式细胞术分析检测外源基因的转导效率和表达水平。采用克隆形成试验检测细胞存活分数。

结果

与对照组相比，碳离子束照射组和γ射线照射组的转导频率分别提高了50 - 83%和5.7 - 38.0%（P<0.05）。与单纯碳离子束、单纯p53以及γ射线联合p53处理相比，1 Gy碳离子束联合p53处理后第1天和第3天p53阳性细胞百分比分别提高了56.0 - 72.0%、63.5 - 82.0%和31.3 - 72.5%（P<0.05）。2 Gy碳离子束联合AdCMV - p53感染组的细胞存活分数降至约2%。