噬菌体phiC31整合酶介导的非洲爪蟾转基因，用于内源性水平的蛋白质表达。
Bacteriophage phiC31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels.
作者信息
Allen Bryan G, Weeks Daniel L
机构信息
Department of Biochemistry, University of Iowa, Iowa City, IA, USA.
出版信息
Methods Mol Biol. 2009;518:113-22. doi: 10.1007/978-1-59745-202-1_9.
Abstract
Bacteriophage phiC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integrase requires no accessory factors, has a high efficiency of recombination, and does not need perfect sequence fidelity for recognition and recombination between these attachment sites. These imperfect attachment sites, or pseudo-attachment sites, are present in many organisms and have been used to insert transgenes in a variety of species. Here we describe the phiC31 integrase approach to make transgenic Xenopus laevis embryos.
摘要
噬菌体phiC31通过整合酶将其基因组插入宿主细菌的基因组中，该整合酶催化噬菌体附着位点（attP）和细菌附着位点（attB）之间的重组。整合酶不需要辅助因子，重组效率高，并且在这些附着位点之间进行识别和重组时不需要完美的序列保真度。这些不完美的附着位点，即假附着位点，存在于许多生物体中，并已被用于在多种物种中插入转基因。在这里，我们描述了phiC31整合酶方法来制备转基因非洲爪蟾胚胎。
相似文献
1
Bacteriophage phiC31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels.
Methods Mol Biol. 2009;518:113-22. doi: 10.1007/978-1-59745-202-1_9.
2
Using phiC31 integrase to make transgenic Xenopus laevis embryos.
Nat Protoc. 2006;1(3):1248-57. doi: 10.1038/nprot.2006.183.
3
Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.
Nat Methods. 2005 Dec;2(12):975-9. doi: 10.1038/nmeth814.
4
Transgene excision in zebrafish using the phiC31 integrase.
Genesis. 2010 Feb;48(2):137-43. doi: 10.1002/dvg.20595.
5
Using ΦC31 integrase to mediate insertion of DNA in Xenopus embryos.
Methods Mol Biol. 2012;917:219-30. doi: 10.1007/978-1-61779-992-1_13.
6
Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by Streptomyces phage phiC31 integrase.
BMC Mol Biol. 2013 Sep 8;14:20. doi: 10.1186/1471-2199-14-20.
7
PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints.
J Gene Med. 2006 Aug;8(8):1008-17. doi: 10.1002/jgm.928.
8
Integration specificity of phage phiC31 integrase in the human genome.
J Mol Biol. 2006 Mar 17;357(1):28-48. doi: 10.1016/j.jmb.2005.11.098. Epub 2005 Dec 22.
9
Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.
Genetics. 2004 Apr;166(4):1775-82. doi: 10.1093/genetics/166.4.1775.
10
Frog transgenesis made simple.
Nat Methods. 2005 Dec;2(12):897-8. doi: 10.1038/nmeth1205-897.
引用本文的文献
1
: Safe harbor landing sites for reproducible and efficient transgenesis in zebrafish.
Sci Adv. 2024 Jun 7;10(23):eadn6603. doi: 10.1126/sciadv.adn6603. Epub 2024 Jun 5.
2
Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.
Front Genet. 2023 Apr 18;14:1085024. doi: 10.3389/fgene.2023.1085024. eCollection 2023.
3
Tbx5 drives expression to regulate a RA-Hedgehog-Wnt gene regulatory network coordinating cardiopulmonary development.
Elife. 2021 Oct 13;10:e69288. doi: 10.7554/eLife.69288.
4
Genome editing for crop improvement: Challenges and opportunities.
GM Crops Food. 2015;6(4):183-205. doi: 10.1080/21645698.2015.1129937.
5
Using ΦC31 integrase to mediate insertion of DNA in Xenopus embryos.
Methods Mol Biol. 2012;917:219-30. doi: 10.1007/978-1-61779-992-1_13.
6
Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).
J Ind Microbiol Biotechnol. 2012 May;39(5):661-72. doi: 10.1007/s10295-011-1069-6. Epub 2011 Dec 13.
7
Xenopus: An emerging model for studying congenital heart disease.
Birth Defects Res A Clin Mol Teratol. 2011 Jun;91(6):495-510. doi: 10.1002/bdra.20793. Epub 2011 Apr 28.
8
A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector.
PLoS One. 2011 Feb 24;6(2):e17267. doi: 10.1371/journal.pone.0017267.
9
Recombinase technology: applications and possibilities.
Plant Cell Rep. 2011 Mar;30(3):267-85. doi: 10.1007/s00299-010-0938-1. Epub 2010 Oct 24.
10
Nanoparticles for retinal gene therapy.
Prog Retin Eye Res. 2010 Sep;29(5):376-97. doi: 10.1016/j.preteyeres.2010.04.004. Epub 2010 May 7.
本文引用的文献
1
PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints.
J Gene Med. 2006 Aug;8(8):1008-17. doi: 10.1002/jgm.928.
2
Phi c31 integrase induces chromosomal aberrations in primary human fibroblasts.
Gene Ther. 2006 Aug;13(15):1188-90. doi: 10.1038/sj.gt.3302789. Epub 2006 May 4.
3
Phage phiC31 integrase-mediated genomic integration of the common cytokine receptor gamma chain in human T-cell lines.
J Gene Med. 2006 May;8(5):646-53. doi: 10.1002/jgm.891.
4
Highly efficient transgenesis in Xenopus tropicalis using I-SceI meganuclease.
Mech Dev. 2006 Feb;123(2):103-13. doi: 10.1016/j.mod.2005.11.006. Epub 2006 Jan 18.
5
Enhancement of plasmid-mediated gene therapy for muscular dystrophy by directed plasmid integration.
Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):419-24. doi: 10.1073/pnas.0504505102. Epub 2005 Dec 30.
6
Transgenic Xenopus laevis embryos can be generated using phiC31 integrase.
Nat Methods. 2005 Dec;2(12):975-9. doi: 10.1038/nmeth814.
7
I-SceI meganuclease-mediated transgenesis in Xenopus.
Dev Dyn. 2006 Jan;235(1):247-52. doi: 10.1002/dvdy.20608.
8
phiC31 integrase confers genomic integration and long-term transgene expression in rat retina.
Invest Ophthalmol Vis Sci. 2005 Jun;46(6):2140-6. doi: 10.1167/iovs.04-1252.
9
In vivo correction of murine hereditary tyrosinemia type I by phiC31 integrase-mediated gene delivery.
Mol Ther. 2005 Mar;11(3):399-408. doi: 10.1016/j.ymthe.2004.11.001.
10
Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31.
Genetics. 2004 Apr;166(4):1775-82. doi: 10.1093/genetics/166.4.1775.
Zotero 插件