噬菌体phiC31整合酶介导的非洲爪蟾转基因，用于内源性水平的蛋白质表达。
Bacteriophage phiC31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels.
作者信息
Allen Bryan G, Weeks Daniel L
机构信息
Department of Biochemistry, University of Iowa, Iowa City, IA, USA.
出版信息
Methods Mol Biol. 2009;518:113-22. doi: 10.1007/978-1-59745-202-1_9.
Abstract
Bacteriophage phiC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integrase requires no accessory factors, has a high efficiency of recombination, and does not need perfect sequence fidelity for recognition and recombination between these attachment sites. These imperfect attachment sites, or pseudo-attachment sites, are present in many organisms and have been used to insert transgenes in a variety of species. Here we describe the phiC31 integrase approach to make transgenic Xenopus laevis embryos.
摘要
噬菌体phiC31通过整合酶将其基因组插入宿主细菌的基因组中，该整合酶催化噬菌体附着位点（attP）和细菌附着位点（attB）之间的重组。整合酶不需要辅助因子，重组效率高，并且在这些附着位点之间进行识别和重组时不需要完美的序列保真度。这些不完美的附着位点，即假附着位点，存在于许多生物体中，并已被用于在多种物种中插入转基因。在这里，我们描述了phiC31整合酶方法来制备转基因非洲爪蟾胚胎。
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2
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Gene Ther. 2006 Aug;13(15):1188-90. doi: 10.1038/sj.gt.3302789. Epub 2006 May 4.
3
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J Gene Med. 2006 May;8(5):646-53. doi: 10.1002/jgm.891.
4
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Mech Dev. 2006 Feb;123(2):103-13. doi: 10.1016/j.mod.2005.11.006. Epub 2006 Jan 18.
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