Claessens J A, Schrier C C, Mockett A P, Jagt E H, Sondermeijer P J
Intervet International BV, Department of Virological Research, Boxmeer, The Netherlands.
J Gen Virol. 1991 Aug;72 ( Pt 8):2003-6. doi: 10.1099/0022-1317-72-8-2003.
The replicative form (RF) DNA of chicken anaemia agent (CAA) was isolated and cloned into bacterial plasmids. After religation of the cloned CAA DNA and transfection into MDCC-MSB1 cells, the DNA could induce c.p.e. characteristic of that caused by CAA, and an antigen was produced which gave positive immunofluorescence when detected with an anti-CAA serum. Sanger sequencing of the 2298 bp genome revealed several open reading frames (ORFs); the major ORF encoded a polypeptide of 51.8K. In SDS-PAGE of CAA viral particles a 50K protein has been reported as the only detectable viral protein. The genomic region downstream of the major ORF had several predicted GC-rich inverted repeats, a poly(A) signal and four copies of an 18 bp repeat element. Database searches did not reveal any sequence with homology to the viral genomic DNA, nor to the amino acid sequence of any of the ORFs, apart from the N-terminal 40 amino acids of the major ORF which showed a limited similarity to the structure of protamines.
鸡贫血因子（CAA）的复制型（RF）DNA被分离出来并克隆到细菌质粒中。将克隆的CAA DNA重新连接并转染到MDCC - MSB1细胞后，该DNA可诱导出CAA所引起的典型细胞病变效应（c.p.e.），并且产生了一种抗原，用抗CAA血清检测时呈现阳性免疫荧光。对2298 bp基因组进行桑格测序揭示了几个开放阅读框（ORF）；主要的ORF编码一个51.8K的多肽。在CAA病毒颗粒的SDS - PAGE分析中，据报道一种50K的蛋白质是唯一可检测到的病毒蛋白。主要ORF下游的基因组区域有几个预测的富含GC的反向重复序列、一个聚腺苷酸（poly(A)）信号和四个18 bp重复元件的拷贝。数据库搜索未发现与病毒基因组DNA或任何ORF的氨基酸序列具有同源性的序列，除了主要ORF的N端40个氨基酸与鱼精蛋白结构有有限的相似性。
