MT1-MMP 缺乏及过表达对角膜基质细胞中血管内皮细胞迁移和增殖的影响。
Effect of MT1-MMP deficiency and overexpression in corneal keratocytes on vascular endothelial cell migration and proliferation.
作者信息
Azar Dimitri T, Casanova Fabio H, Mimura Tatsuya, Jain Sandeep, Chang Jin-Hong
机构信息
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
出版信息
Curr Eye Res. 2008 Nov;33(11):954-62. doi: 10.1080/02713680802461106.
Abstract
PURPOSE
To determine the effect of keratocyte-derived MT1-MMP on calf pulmonary artery endothelial cell (CPAE) proliferation and migration.
METHODS
Keratocyte lines were generated from MT1-MMP knockout (KO) and wild type (WT) mice. WT keratocytes were transfected with WT or mutant MT1-MMP DNAs (DeltaTC or E240A). The effect of keratocyte-conditioned media on CPAE proliferation and migration was assayed.
RESULTS
KO keratocyte conditioned media resulted in the greatest increase of CPAE cell proliferation (190.5+/-6.0%; p<0.01). WT keratocyte conditioned media showed higher CPAE proliferation (155.4+/-3.6%) than WT/MT1-MMP-transfected keratocytes (119.7+/-2.2%; p<0.001). Migration assays confirmed these findings.
CONCLUSIONS
Keratocyte-derived MT1-MMP has anti-angiogenic effects in CPAE cells.
摘要
目的
确定角膜细胞源性基质金属蛋白酶-1（MT1-MMP）对小牛肺动脉内皮细胞（CPAE）增殖和迁移的影响。
方法
从MT1-MMP基因敲除（KO）小鼠和野生型（WT）小鼠中生成角膜细胞系。用野生型或突变型MT1-MMP DNA（ΔTC或E240A）转染野生型角膜细胞。检测角膜细胞条件培养基对CPAE增殖和迁移的影响。
结果
KO角膜细胞条件培养基导致CPAE细胞增殖增加最多（190.5±6.0%；p<0.01）。野生型角膜细胞条件培养基显示出比野生型/MT1-MMP转染的角膜细胞更高的CPAE增殖（155.4±3.6%）（119.7±2.2%；p<0.001）。迁移试验证实了这些发现。
结论
角膜细胞源性MT1-MMP对CPAE细胞具有抗血管生成作用。
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