Calli Baris, Chung Li-Ching, Arslan Doga, Vanbroekhoven Karolien
Environmental Engineering Department, Faculty of Engineering, Marmara University, Goztepe, Istanbul, Turkey.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009 Jan;44(1):78-86. doi: 10.1080/10934520802515418.
The effect of pH on dark fermentative H(2) production at 55 degrees C was studied using three different inocula namely windrow yard waste compost (G), anaerobic organic waste compost (H) and activated sludge (A) and 2 g/L glucose as substrate. The sequential batch experiments were performed by controlling the pH at 5, 5.5 and 6. The highest molar H(2) yields were found to be 1.7 at pH 5 for G and H and 1.6 at pH 5.5 for A, while severe drops in the subsequent runs were associated with lactate fermentation. At pH 5.5 in G, despite the butyrate formation ceased, lower but more stable H(2) yields of about 1.1 were obtained through acetate-ethanol fermentation. Besides, the clostridial thermopiles prevalent at pH 5 were substituted by Thermoanaerobacterium-related species at pH 5.5 and 6. The findings suggested that for a stable H(2) yield through acetate-butyrate fermentation at 55 degrees C with compost inoculum G or H, the pH has to be controlled between pH 5 and 5.5. This is one of the few papers discussing the effects of pH on H(2) production through thermophilic mixed fermentation of glucose by using three different natural mixed culture inocula.
使用三种不同的接种物，即堆肥场废弃物堆肥（G）、厌氧有机废弃物堆肥（H）和活性污泥（A），以及2 g/L葡萄糖作为底物，研究了pH对55℃下暗发酵产氢的影响。通过将pH控制在5、5.5和6进行序批式实验。发现G和H在pH 5时的最高氢气摩尔产率为1.7，A在pH 5.5时的最高氢气摩尔产率为1.6，而后续运行中的产率严重下降与乳酸发酵有关。在G中pH 5.5时，尽管丁酸形成停止，但通过乙酸-乙醇发酵获得了约1.1的较低但更稳定的氢气产率。此外，在pH 5时普遍存在的梭菌嗜热菌群在pH 5.5和6时被嗜热栖热菌相关物种取代。研究结果表明，对于使用堆肥接种物G或H在55℃下通过乙酸-丁酸发酵实现稳定的氢气产率，pH必须控制在5至5.5之间。这是少数几篇讨论pH对使用三种不同天然混合培养接种物通过葡萄糖嗜热混合发酵产氢影响的论文之一。
