Akkasaeng Chutipong, Tantisuwichwong Napaporn, Chairam Issariya, Prakrongrak Narumon, Jogloy Sanun, Pathanothai Aran
Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Pak J Biol Sci. 2007 May 15;10(10):1611-7. doi: 10.3923/pjbs.2007.1611.1617.
Water deficits trigger signaling cascades leading to modulation of protein expression in plant tissues. Identification of peanut leaf proteins regulated by water stress provides some insights of cellular and molecular response of peanut plants to drought stress. Peanut variety Khon Kaen 4, a water-stress sensitive variety, was grown in a growth chamber under controlled environment. Water stress was imposed on day 30 after seedling emergence by withholding watering peanut plants for 6 days as compared to plants adequately supplied with water. Total protein were prepared from a leaflet of fully expanded leaf on the main stem. Proteins were separated in duplicated gels using two-dimensional gel electrophoresis and visualized by silver nitrate staining. Image analysis was performed using ImageMaster 2D Platinum 5.0 to determine proteins regulated by water stress. Molecular mass and isoelectric point of each regulated protein were used in database queries for protein identification. One protein was induced under water stress and the homologous protein was identified as Serine/threonine-protein phosphatase PP 1. Five proteins were down-regulated by water deficit. The homologous proteins were chaperone protein DNAJ, auxin-responsive protein IAA29, peroxidase 43, caffeoyl-CoA O-methyltransferase and SNF1-related protein kinase regulatory subunit beta-2. Down-regulated proteins may be associated with sensitivity of the peanut variety to water stress.
水分亏缺引发信号级联反应，导致植物组织中蛋白质表达的调节。鉴定受水分胁迫调节的花生叶片蛋白质，可为花生植株对干旱胁迫的细胞和分子反应提供一些见解。花生品种孔敬4号是一个对水分胁迫敏感的品种，在可控环境的生长室中种植。与充分供水的植株相比，在幼苗出土后第30天对花生植株停止浇水6天，从而施加水分胁迫。从主茎上完全展开的叶片的小叶中制备总蛋白。使用二维凝胶电泳在重复的凝胶中分离蛋白质，并通过硝酸银染色进行可视化。使用ImageMaster 2D Platinum 5.0进行图像分析，以确定受水分胁迫调节的蛋白质。每个受调节蛋白质的分子量和等电点用于数据库查询以鉴定蛋白质。一种蛋白质在水分胁迫下被诱导，其同源蛋白质被鉴定为丝氨酸/苏氨酸蛋白磷酸酶PP 1。五种蛋白质因水分亏缺而下调。同源蛋白质分别为伴侣蛋白DNAJ、生长素响应蛋白IAA29、过氧化物酶43、咖啡酰辅酶A O-甲基转移酶和SNF1相关蛋白激酶调节亚基β-2。下调的蛋白质可能与花生品种对水分胁迫的敏感性有关。
