Sangyoka Suksaman, Reungsang Alissara, Moonamart Samart
Rachjabaht Phiboonsongkram University, Phitsanuloke, 65000 Thailand.
Pak J Biol Sci. 2007 Jun 1;10(11):1782-9. doi: 10.3923/pjbs.2007.1782.1789.
Anaerobic hydrogen production from cassava wastewater by heat-treated UASB granules was conducted in a 10 L bioreactor with a working volume of 8 L at room temperature and pH 6.0 by batch and repeated-batch fermentations. Specific hydrogen production potential, hydrogen yield and the maximum hydrogen production rate of 39, 304.81 mL, 0.22 mL mg-COID(-1) and 851.84 mL h(-1), respectively, were obtained in a batch reactor. A repeated-batch was conducted when the glucose concentration in fermentative broth was depleted to 150-250 mg L(-1) which equivalent to 10-15% of initial glucose concentration. Repeated-batch reactor was operated at 3 different feed-in/feed-out rates i.e., 25, 50 and 75%. Results revealed that a suitable feed-in/feed out rate for production of hydrogen from cassava wastewater was at 75%. This was indicated by the highest hydrogen yield, the highest potential maximal amount of hydrogen produced, a relatively high maximum hydrogen production rate, a relatively high maximum specific hydrogen production rate and a relatively short lag time of 0.0094 mL mg-COD(-1), 12,532.80 mL, 540.46 mL h(-1), 3.5 mL g-VSS(-1) h and 5.31 h, respectively. Major soluble metabolites were acetic and butyric acids. Our results indicated that repeated batch fermentation was more effective in producing hydrogen from cassava wastewater than batch fermentation.
在室温及pH值为6.0的条件下，采用间歇式和重复间歇式发酵，在一个工作体积为8L的10L生物反应器中，利用经热处理的上流式厌氧污泥床（UASB）颗粒对木薯废水进行厌氧产氢。在间歇式反应器中，分别获得了39、304.81mL、0.22mL mg-COD⁻¹和851.84mL h⁻¹的比产氢潜力、氢气产率和最大产氢速率。当发酵液中的葡萄糖浓度降至150 - 250mg L⁻¹（相当于初始葡萄糖浓度的10 - 15%）时，进行重复间歇式发酵。重复间歇式反应器以25%、50%和75%这3种不同的进料/出料速率运行。结果表明，木薯废水产氢的合适进料/出料速率为75%。这表现为最高的氢气产率、最高的潜在最大产氢量、相对较高的最大产氢速率、相对较高的最大比产氢速率以及相对较短的延迟时间，分别为0.0094mL mg-COD⁻¹、12532.80mL、540.46mL h⁻¹、3.5mL g-VSS⁻¹ h和5.31h。主要的可溶性代谢产物为乙酸和丁酸。我们的结果表明，重复间歇式发酵在木薯废水产氢方面比间歇式发酵更有效。
