酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）检测沙特奶牛群亚临床副结核病的效率

The efficiency of ELISA and PCR in detecting subclinical paratuberculosis in the Saudi dairy herds.

作者信息

Al Hajri S M, Alluwaimi A M

机构信息

Division of Animal Health, Ministry of Agriculture, Al Ahsaa, Saudi Arabia.

出版信息

Pak J Biol Sci. 2007 Jun 1;10(11):1906-9. doi: 10.3923/pjbs.2007.1906.1909.

DOI:10.3923/pjbs.2007.1906.1909

PMID:19086558

Abstract

This study was aimed to reveal the presence of John's disease in the Saudi dairy herds using the newly developed diagnostic tests, ELISA and PCR. A total of 687 serum and fecal samples were collected from dairy cattle of four different ages, one, two, four and six years age cattle of three geographically different dairy farms. IDEXX ELISA revealed 15 (2%) positive samples and 17 (2.5%) samples were inconclusive. PCR test were used only to test 62 ELISA-negative samples that their OD readings were the highest and all the inconclusive samples. The PCR disclosed more positive samples (22/62 = 32%), interestingly among the samples of the two-year old cattle. The study has conclusively confirmed the presence of the disease in the Saudi dairy herds. It has also approved the effectiveness of ELISA and PCR tests in revealing the MAP infection at the subclinical stage.

摘要

本研究旨在使用新开发的诊断测试（酶联免疫吸附测定法（ELISA）和聚合酶链反应（PCR））揭示沙特奶牛群中副结核的存在情况。从三个地理位置不同的奶牛场中四个不同年龄段（一岁、两岁、四岁和六岁）的奶牛身上总共采集了687份血清和粪便样本。IDEXX ELISA检测出15份（2%）阳性样本，17份（2.5%）样本结果不确定。PCR测试仅用于检测62份ELISA阴性且光密度读数最高的样本以及所有结果不确定的样本。PCR检测出了更多阳性样本（22/62 = 32%），有趣的是，这些阳性样本来自两岁奶牛的样本中。该研究最终证实了沙特奶牛群中存在这种疾病。它还证明了ELISA和PCR测试在揭示亚临床阶段副结核分枝杆菌感染方面的有效性。