[气相色谱-质谱联用-模式识别法用于当归质量控制的研究]
[Development of gas chromatographic-mass spectrometry-pattern recognition method for the quality control of Chinese Angelica].
作者信息
Piao Xiang-lan, Hong Cheng-quan
机构信息
China Minority Traditional Medicine Center, Central University for Nationalities, Beijing 100081, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Aug;33(16):2008-10.
OBJECTIVE
To compare 39 root samples of Angelica sinensis and A. acutiloba from China and Japan for the quality control of Chinese Angelica.
METHOD
An HP-5 (0.32 mm x 30 m, 0.25 microm) column was used for the GC-MS analysis. The oven temperature was programmed from 120 degrees C to 280 degrees C at a rate of 5 degrees C x min(-1). Using principal component analysis, cluster analysis, and discriminant analysis on the sample fingerprints for chemical pattern recognition research.
RESULT
Z-Ligustilide was the key principle distinguishing Chin samples from Japan. Moreover, using discriminant analysis, seven samples (four of A. sinensis, three of A. acutiloba) were validated. All samples tested were successfully classified according to their species origin.
目的
比较来自中国和日本的39份当归和东当归根样本，以用于当归的质量控制。
方法
采用HP-5（0.32毫米×30米，0.25微米）色谱柱进行气相色谱-质谱分析。柱温箱温度以5℃×分钟⁻¹的速率从120℃程序升温至280℃。运用主成分分析、聚类分析和判别分析对样本指纹图谱进行化学模式识别研究。
结果
Z-藁本内酯是区分中国和日本样本的关键成分。此外，通过判别分析，验证了7个样本（4份当归、3份东当归）。所有测试样本均根据其物种来源成功分类。
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