米非司酮作为一种抗着床避孕药：在着床期对子宫自然杀伤细胞的调节作用。

Mifepristone as an anti-implantation contraceptive drug: roles in regulation of uterine natural killer cells during implantation phase.

作者信息

Zhu Hong-Xia, Zhang Wu-Wen, Zhuang Ya-Ling, Huang Li-Li

机构信息

Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

出版信息

Am J Reprod Immunol. 2009 Jan;61(1):68-74. doi: 10.1111/j.1600-0897.2008.00664.x.

DOI:10.1111/j.1600-0897.2008.00664.x

PMID:19086994

Abstract

PROBLEM

To investigate the immunological mechanism of low-dose mifepristone acting as a contraceptive at the level of the endometrium.

METHOD OF STUDY

Endometrial explants were cultured in vitro with or without mifepristone treatment for 24 hr. Some tissues were fixed and immunostained for CD56, while other tissues were dissociated and cells analysed by three colour flow cytometry for CD3, CD56 and CD16.

RESULTS AND CONCLUSION

Results showed a significant increase in the number of CD56(+) natural killer (NK) cells and the percentages of CD3(-) CD56(+) CD16(-) NK cell subset in the tissue treated with mifepristone, while the percentage of CD3(-) CD56(+) CD16(+) NK cell subset remained unaffected. It shows that low-dose mifepristone increases the number of CD56(+) NK cells and the percentage of CD3(-) CD56(+) CD16(-) NK subset in receptive endometrium and provides new insights into the immunological mechanism of low-dose mifepristone as an anti-implantation contraceptive drug.

摘要

问题

研究低剂量米非司酮在子宫内膜水平作为避孕药发挥作用的免疫机制。

研究方法

将子宫内膜外植体在有或无米非司酮处理的情况下体外培养24小时。一些组织进行固定并对CD56进行免疫染色，而其他组织进行解离，细胞通过三色流式细胞术分析CD3、CD56和CD16。

结果与结论

结果显示，用米非司酮处理的组织中CD56(+)自然杀伤（NK）细胞数量显著增加，CD3(-)CD56(+)CD16(-)NK细胞亚群的百分比增加，而CD3(-)CD56(+)CD16(+)NK细胞亚群的百分比未受影响。这表明低剂量米非司酮增加了接受性子宫内膜中CD56(+)NK细胞的数量和CD3(-)CD56(+)CD16(-)NK亚群的百分比，并为低剂量米非司酮作为抗着床避孕药的免疫机制提供了新的见解。