Régeard M, Trotard M, Lepère C, Gripon P, Le Seyec J
Institut National de la Santé Et de la Recherche Médicale, Unité 522, Rennes, France.
J Viral Hepat. 2008 Dec;15(12):865-70. doi: 10.1111/j.1365-2893.2008.01048.x.
Entry of the hepatitis C virus (HCV) into the cell seems to be a complex multi-step process involving several cellular factors such as the scavenger class B type I receptor (SRBI). Until now, all investigations conducted to assess the involvement of SRBI have been based on in vitro infection models which use human hepatoma-derived cell lines. However, the HCV entry pathway may be altered in these types of cells because of the impairment of some hepatic characteristics. In this study, we showed that SRBI also plays an essential role in HCV entry into primary human hepatocytes with two distinct approaches: gene extinction and antibodies neutralization assays.
丙型肝炎病毒（HCV）进入细胞似乎是一个复杂的多步骤过程，涉及多种细胞因子，如B类I型清道夫受体（SRBI）。到目前为止，所有评估SRBI参与情况的研究都是基于使用人肝癌衍生细胞系的体外感染模型。然而，由于某些肝脏特性的受损，在这些类型的细胞中HCV进入途径可能会发生改变。在本研究中，我们通过两种不同的方法——基因敲除和抗体中和试验，表明SRBI在HCV进入原代人肝细胞的过程中也起着至关重要的作用。
