对白斑综合征病毒中一种参与病毒潜伏的新型E3泛素连接酶的鉴定与特性分析。
Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency.
作者信息
He Fang, Kwang Jimmy
机构信息
Animal Health Biotechnology, Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
出版信息
Virol J. 2008 Dec 17;5:151. doi: 10.1186/1743-422X-5-151.
White spot syndrome virus (WSSV) is one major pathogen in shrimp aquaculture. WSSV ORF403 is predicted to encode a protein of 641 amino acids, which contains a C3H2C2 RING structure. In the presence of an E2 conjugating enzyme from shrimp, WSSV403 can ubiquitinate itself in vitro, indicating it can function as a viral E3 ligase. Besides, WSSV403 E3 ligase can be activated by a series of E2 variants. Based on RT-PCR and Real time PCR, we detected transcription of WSSV403 in the commercial specific-pathogen-free (SPF) shrimp, suggesting its role as a latency-associated gene. Identified in yeast two-hybrid screening and verified by pull-down assays, WSSV403 is able to bind to a shrimp protein phosphatase (PPs), which was characterized before as an interaction partner for another latent protein WSSV427. Our studies suggest that WSSV403 is a regulator of latency state of WSSV by virtue of its E3 ligase function.
白斑综合征病毒（WSSV）是对虾养殖业中的一种主要病原体。WSSV的ORF403预计编码一种含有641个氨基酸的蛋白质，该蛋白质含有一个C3H2C2型泛素连接酶结构域。在存在来自对虾的E2缀合酶的情况下，WSSV403能够在体外进行自身泛素化，这表明它可以作为一种病毒E3连接酶发挥作用。此外，WSSV403 E3连接酶可以被一系列E2变体激活。基于逆转录-聚合酶链反应（RT-PCR）和实时荧光定量聚合酶链反应（Real time PCR），我们在商业化的无特定病原体（SPF）对虾中检测到了WSSV403的转录，这表明它作为一个潜伏相关基因发挥作用。在酵母双杂交筛选中鉴定并通过下拉试验验证，WSSV403能够与一种对虾蛋白磷酸酶（PPs）结合，该蛋白磷酸酶之前被鉴定为另一种潜伏蛋白WSSV427的相互作用伴侣。我们的研究表明，WSSV403凭借其E3连接酶功能，是WSSV潜伏状态的一个调节因子。
Zotero 插件