Shekar Malathi, Venugopal Moleyur Nagarajappa
Department of Fisheries Microbiology, DBT Bioinformatics Centre, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, 575 002, India.
Arch Virol. 2019 Apr;164(4):961-969. doi: 10.1007/s00705-019-04150-y. Epub 2019 Jan 28.
White spot syndrome virus (WSSV), is a major viral pathogen affecting the shrimp culture industry worldwide. Studies in understanding the mechanisms of WSSV pathogenicity has led to the identification of The Really Interesting New Gene (RING) finger domains in WSSV encoded proteins that have been shown to function as E3 ligase modulating the host-ubiquitin pathway. In this study, we report two proteins encoded by the WSSV genome to harbor a double zinc finger domain, one each in its N- and C-terminal region. Sequence and structural analysis of the two domains showed the N- and C-terminal domains to be similar to known RING1 and RING2 domains of eukaryotic RBR (RING-between-RING) ligases respectively. This is the first report wherein genes within WSSV are shown to encode for double RING domains, which could pave way in understanding further, the function of these proteins and their role in the pathogenic mechanisms of the virus.
白斑综合征病毒（WSSV）是影响全球对虾养殖业的主要病毒病原体。对WSSV致病机制的研究已导致在WSSV编码蛋白中鉴定出真有趣新基因（RING）结构域，这些结构域已被证明可作为E3连接酶调节宿主泛素途径。在本研究中，我们报道了WSSV基因组编码的两种蛋白含有双锌指结构域，分别位于其N端和C端区域。对这两个结构域的序列和结构分析表明，N端和C端结构域分别类似于真核RBR（RING-中间-RING）连接酶的已知RING1和RING2结构域。这是首次报道WSSV内的基因编码双RING结构域，这可能为进一步了解这些蛋白的功能及其在病毒致病机制中的作用铺平道路。
