不明原因复发性自然流产患者蜕膜及外周血CD4⁺CD25⁺调节性T细胞比例的变化
[Changes in proportion of decidua and peripheral blood CD(4)(+)CD(25)(+) regulatory T cells in unexplained recurrent spontaneous abortion patients].
作者信息
Yang Hui, Lin Qi-de, Qiu Li-Hua, Zhao Ai-Ming, Hu Ke, Chen Guang-Jie, Shi Gui-Ying
机构信息
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 2008 Aug;43(8):602-5.
OBJECTIVE
To study the changes in the percentage of CD(4)(+)CD(25)(+) regulatory T (Tr) cells in peripheral blood and deciduas in unexplained recurrent spontaneous abortion (URSA) patients, normal non-pregnant and pregnant women respectively.
METHODS
The percentage of CD(4)(+)CD(25)(+) Tr cells in deciduas and peripheral blood from 25 URSA patients, 22 normal non-pregnant (NNP) women, and 34 normal early pregnant (NP) women were measured by double-staining followed by flow cytometric analysis.
RESULTS
(1) The percentage of CD(4)(+)CD(25)(bright) T cells in peripheral blood in both URSA and NP [(1.55 +/- 0.77)%, (2.65 +/- 1.10)%, respectively] women were increased significantly than that in NNP women [(0.39 +/- 0.14)%, P < 0.05]. The percentage of CD(4)(+)CD(25)(bright) T cells in peripheral blood in URSA women was significantly lower than that in NP women (P < 0.05). (2) The percentage of CD(4)(+)CD(25)(bright) T cells in decidua in URSA women was significantly lower than that in NP women [(0.59 +/- 0.23)%, (1.24 +/- 0.55)%, respectively, P < 0.01]. There was no significant difference in the percentage of CD(4)(+)CD(25)(dim) T cells in decidua between URSA women and NP women [(4.23 +/- 1.52)%, (3.75 +/- 1.88)%, respectively, P > 0.05]. (3) The proportion of CD(4)(+)CD(25)(bright)/CD(4)(+) cells in deciduas was significantly higher than that in peripheral blood in NP women [(13.10 +/- 10.25)%, (5.59 +/- 2.62)%, respectively, P < 0.05]. However, a significant difference in the proportion of CD(4)(+)CD(25)(bright)/CD(4)(+) between decidua and peripheral blood was not found in URSA patients [(5.16 +/- 2.83)%, (4.64 +/- 2.07)%, respectively, P > 0.05)].
CONCLUSIONS
The number of CD(4)(+)CD(25)(+) Tr cells is increased in normal pregnancy and decreased in URSA. Therefore, CD(4)(+)CD(25)(+) Tr cells may play an important role in maintaining maternal-fetal tolerance and may be involved in the pathogenesis of URSA.
目的
分别研究不明原因复发性自然流产（URSA）患者、正常未孕女性及正常孕早期女性外周血和蜕膜中CD4⁺CD25⁺调节性T（Tr）细胞百分比的变化。
方法
采用双色免疫荧光标记及流式细胞术分析，检测25例URSA患者、22例正常未孕（NNP）女性及34例正常孕早期（NP）女性蜕膜和外周血中CD4⁺CD25⁺Tr细胞的百分比。
结果
（1）URSA组和NP组女性外周血中CD4⁺CD25⁺亮型T细胞百分比[分别为（1.55±0.77）%、（2.65±1.10）%]均显著高于NNP组女性[（0.39±0.14）%，P<0.05]。URSA组女性外周血中CD4⁺CD25⁺亮型T细胞百分比显著低于NP组女性（P<0.05）。（2）URSA组女性蜕膜中CD4⁺CD25⁺亮型T细胞百分比显著低于NP组女性[分别为（0.59±0.23）%、（1.24±0.55）%，P<0.01]。URSA组女性与NP组女性蜕膜中CD4⁺CD25⁺暗型T细胞百分比[分别为（4.23±1.52）%、（3.75±1.88）%]差异无统计学意义（P>0.05）。（3）NP组女性蜕膜中CD4⁺CD25⁺亮型/CD4⁺细胞比例显著高于外周血[分别为（13.10±10.25）%、（5.59±2.62）%，P<0.05]。然而，URSA患者蜕膜与外周血中CD4⁺CD25⁺亮型/CD4⁺细胞比例差异无统计学意义[分别为（5.16±2.83）%、（4.64±2.07）%，P>0.05]。
结论
正常妊娠时CD4⁺CD25⁺Tr细胞数量增加，URSA时减少。因此，CD4⁺CD25⁺Tr细胞可能在维持母胎耐受中起重要作用，并可能参与URSA的发病机制。
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