[不明原因复发性自然流产中调节性T细胞比例及功能的测定]

[The measurement of proportion and function of regulatory T cells in unexplained recurrent spontaneous abortion].

作者信息

Liu Yu-sheng, Zhao Shu-yun, Ji Jing-juan, Zhai Zhi-min, Tong Xian-hong, Luo Li-hua, Luan Hong-bing

机构信息

Reproductive Medicine Center Provincial Hospital, Affiliated to Anhui Medical University, Hefei 230001, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2007 Mar;42(3):176-9.

PMID:17537303

Abstract

OBJECTIVE

To investigate the proportion and function of CD(4)(+)CD(25)(+) regulatory T cells (CD(4)(+)CD(25)(+) Tr) in unexplained recurrent spontaneous abortion (URSA).

METHODS

(1) Proportion measurement: the proportion of CD(4)(+)CD(25)(+) Tr cells in peripheral blood was measured by double-label flow cytometric analysis. The samples were taken from 15 URSA women, 15 normal non-pregnancy women and 13 normal pregnancy women. (2) Function measurement: CD(4)(+)CD(25)(+) Tr cells and CD(4)(+)CD(25)(-) T cells were extracted from peripheral blood lymphocytes by the microbeads separation. The purity of CD(4)(+)CD(25)(+) Tr cells and CD(4)(+)CD(25)(-) T cells was measured by flow cytometry. The growth inhibitory effect of CD(4)(+)CD(25)(+) Tr cells on CD(4)(+)CD(25)(-) T cells was assessed in vitro.

RESULTS

The proportion of CD(4)(+)CD(25)(+) Tr cells was decreased significantly in URSA women (6.9 +/- 1.8)% than that in normal non-pregnancy women [(10.8 +/- 1.1)%] (P<0.05) and normal pregnancy women [(11.2 +/- 1.4)%] (P<0.01). Moreover, the suppressive rate of CD(4)(+)CD(25)(+) Tr cells was decreased significantly in URSA women (75 +/- 6)% than that in normal non-pregnancy women [(89 +/- 4)%] (P<0.05) and normal pregnancy women [(90 +/- 4)%] (P<0.01). However, with respect to the proportion and function of CD(4)(+)CD(25)(+) Tr cells, there was no significant difference between normal non-pregnancy women and normal pregnancy women (P > 0.05).

CONCLUSION

The results suggest that decrease in proportion and function of CD(4)(+)CD(25)(+) Tr cells may be associated with URSA.

摘要

目的

探讨CD4⁺CD25⁺调节性T细胞（CD4⁺CD25⁺Tr）在不明原因复发性自然流产（URSA）中的比例及功能。

方法

（1）比例测定：采用双标记流式细胞术分析检测外周血中CD4⁺CD25⁺Tr细胞的比例。样本取自15例URSA患者、15例正常非妊娠女性和13例正常妊娠女性。（2）功能测定：通过微珠分离法从外周血淋巴细胞中提取CD4⁺CD25⁺Tr细胞和CD4⁺CD25⁻T细胞。采用流式细胞术检测CD4⁺CD25⁺Tr细胞和CD4⁺CD25⁻T细胞的纯度。体外评估CD4⁺CD25⁺Tr细胞对CD4⁺CD25⁻T细胞的生长抑制作用。

结果

URSA患者CD4⁺CD25⁺Tr细胞比例（6.9±1.8）%显著低于正常非妊娠女性[（10.8±1.1）%]（P<0.05）和正常妊娠女性[（11.2±1.4）%]（P<0.01）。此外，URSA患者CD4⁺CD25⁺Tr细胞的抑制率（75±6）%显著低于正常非妊娠女性[（89±4）%]（P<0.05）和正常妊娠女性[（90±4）%]（P<0.01）。然而，关于CD4⁺CD25⁺Tr细胞的比例和功能，正常非妊娠女性和正常妊娠女性之间无显著差异（P>0.05）。