双氢青蒿素对上皮性卵巢癌细胞系有丝分裂原活化蛋白激酶增殖和磷酸化的影响

[Effects of dihydroartiminisin on proliferation and phosphorylation of mitogen-activated protein kinase in epithelial ovarian cancer cell lines].

作者信息

Tan Xian-Jie, Plouet Jean, Lang Jing-He, Wu Ming, Shen Keng

机构信息

Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2008 Sep;43(9):662-5.

PMID:19087515

Abstract

OBJECTIVE

To determine the effect of dihydroartiminisin on the proliferation and phosphorylation of mitogen-activated protein kinase (MAPK) in SKOV3 and OVCAR3 ovarian cancer cell lines.

METHODS

Methyl thiazolyl tetrazolium assay was performed to evaluate the anti-proliferative effect of dihydroartiminisin in SKOV3 and OVCAR3 cells, and Western blot was used to determine its effect on phosphorylation level of MAPK, including extra-cell regulated kinase (ERK) 1/2 and p38 protein kinase, in the two cell lines.

RESULTS

Dihydroartiminisin inhibited the proliferation of ovarian cancer cells in vitro, with a mean of 50% inhibition concentration (IC(50)) at 72 h of (9.0 +/- 1.4) micromol/L for SKOV3 and (5.5 +/- 1.2) micromol/L for OVCAR3 respectively. Compared to cells without dihydroartiminisin treatment, phosphorylation level of ERK 1/2 in SKOV3 and OVCAR3 cells treated with dihydroartiminisin decreased by 64.2% and 75.3% respectively (P < 0.05), while phosphorylation of p38 protein kinase in SKOV3 and OVCAR3 only decreased by 8.5%and 6.4%respectively (P > 0.05).

CONCLUSION

Dihydroartiminisin can inhibit the proliferation of ovarian cancer cell in vitro, probably through down-regulation of the phosphorylation of ERK 1/2 in ovarian cancer cells.

摘要

目的

确定双氢青蒿素对SKOV3和OVCAR3卵巢癌细胞系中丝裂原活化蛋白激酶（MAPK）增殖和磷酸化的影响。

方法

采用甲基噻唑基四氮唑蓝法评估双氢青蒿素对SKOV3和OVCAR3细胞的抗增殖作用，并用蛋白质免疫印迹法测定其对这两种细胞系中MAPK磷酸化水平的影响，包括细胞外调节激酶（ERK）1/2和p38蛋白激酶。

结果

双氢青蒿素在体外抑制卵巢癌细胞的增殖，在72小时时，SKOV3细胞的平均半数抑制浓度（IC50）为（9.0±1.4）μmol/L，OVCAR3细胞为（5.5±1.2）μmol/L。与未用双氢青蒿素处理的细胞相比，用双氢青蒿素处理的SKOV3和OVCAR3细胞中ERK 1/2的磷酸化水平分别降低了64.2%和75.3%（P<0.05），而SKOV3和OVCAR3中p38蛋白激酶的磷酸化仅分别降低了8.5%和6.4%（P>0.05）。