Zhang Shuqun, Ma Yinan, Jiang Jiantao, Dai Zhijun, Gao Xiaoyan, Yin Xiaoran, Xi Wentao, Min Weili
Department of Oncology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
Oncol Lett. 2014 May;7(5):1375-1380. doi: 10.3892/ol.2014.1918. Epub 2014 Feb 27.
The aim of the present study was to investigate the inhibitory effects of dihydroartemisinin (DHA) on the primary tumor growth and metastasis of the human breast cancer cell line, MDA-MB-231, . The expression levels of urokinase-type plasminogen activator (uPA) were detected by immunocytochemistry in two cell lines (MCF-7 and MDA-MB-231). The MDA-MB-231 cell activity was inhibited by various concentration gradients of DHA. The inhibitory rate, cell growth curve and apoptotic morphological observations were obtained using the MTT assay at 0, 24, 48 and 72 h. Cell scratch migration was performed at various time-points to test the cell proliferation and migration capacity. Reverse transcription-polymerase chain reaction was used to analyze the effect of DHA on uPA mRNA expression in breast cancer cells. The human breast cancer cell line, MDA-MB-231, possesses higher metastatic potential and relatively higher expression of uPA when compared with the MCF-7 cell line. DHA was found to inhibit the proliferation and migration capacity of the cell line, MDA-MB-231, . The growth inhibition occurred in a time- and dose-dependent manner, with IC values of 117.76±0.04, 60.26±0.12 and 52.96±0.07 μmol/l following 24, 48 and 72 h, respectively. The inhibition of uPA was observed to decrease breast cancer cell growth and migration. Thus, results of the present study indicate that DHA may be used for further studies with regard to breast cancer therapy.
本研究的目的是探讨双氢青蒿素（DHA）对人乳腺癌细胞系MDA-MB-231原发性肿瘤生长和转移的抑制作用。通过免疫细胞化学检测两种细胞系（MCF-7和MDA-MB-231）中尿激酶型纤溶酶原激活剂（uPA）的表达水平。不同浓度梯度的DHA抑制了MDA-MB-231细胞活性。在0、24、48和72小时使用MTT法获得抑制率、细胞生长曲线和凋亡形态观察结果。在不同时间点进行细胞划痕迁移实验以检测细胞增殖和迁移能力。采用逆转录-聚合酶链反应分析DHA对乳腺癌细胞中uPA mRNA表达的影响。与MCF-7细胞系相比，人乳腺癌细胞系MDA-MB-231具有更高的转移潜能和相对较高的uPA表达。发现DHA抑制细胞系MDA-MB-231的增殖和迁移能力。生长抑制呈时间和剂量依赖性，在24、48和72小时后的IC值分别为117.76±0.04、60.26±0.12和52.96±0.07μmol/L。观察到对uPA的抑制可降低乳腺癌细胞的生长和迁移。因此，本研究结果表明DHA可用于乳腺癌治疗的进一步研究。
