[参与牙周病的古菌的鉴定与定量分析]

[Identification and quantitative analysis of Archaea involved in periodontal disease].

作者信息

Li Chao-lun, Jiang Yun-tao, Zhang Ming-zhu, Liang Jing-ping

机构信息

Department of Oral Medicine, School of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 Oct;43(10):589-91.

PMID:19087619

Abstract

OBJECTIVE

To make qualitative and quantitative analysis of Archaea in subgingival plaque sample and to investigate the relationship between periodontal disease and Archaea.

METHODS

Subgingival plaque was collected from 23 patients with aggressive periodontitis, 29 with chronic periodontitis, 35 with plaque-induced gingivitis and 38 healthy controls. Qualitative and quantitative analysis of methanogenic archaea was performed by amplification of the 16S rRNA genes in the DNA extracted from the plaque samples.

RESULTS

Archaea were found in 65% of aggressive periodontitis patients, 72% of chronic periodontitis, 26% of gingivitis and zero of healthy subjects. Quantitative analysis showed the average abundance of archaeal 16S rRNA gene in Archaea-positive patients was different among the three groups. The average 16S rRNA gene copy number from per microg wet plaque was 6.66 x 10(6) in aggressive periodontitis sufferers, 4.47 x 10(6) in chronic periodontitis and 1.78 x 10(6) in gingivitis groups. The prevalence of Archaea and the average Archaea 16S rRNA gene numbers in periodontitis groups were higher than those in gingivitis group (P < 0.05).

CONCLUSIONS

This suggests that Archaea may be implicated as causative agents for periodontitis.

摘要

目的

对龈下菌斑样本中的古菌进行定性和定量分析，并研究牙周病与古菌之间的关系。

方法

收集23例侵袭性牙周炎患者、29例慢性牙周炎患者、35例菌斑性牙龈炎患者和38例健康对照者的龈下菌斑。通过扩增从菌斑样本中提取的DNA中的16S rRNA基因，对产甲烷古菌进行定性和定量分析。

结果

在65%的侵袭性牙周炎患者、72%的慢性牙周炎患者、26%的牙龈炎患者中发现了古菌，而健康受试者中未发现。定量分析显示，古菌阳性患者中古菌16S rRNA基因的平均丰度在三组之间存在差异。侵袭性牙周炎患者每微克湿菌斑中16S rRNA基因的平均拷贝数为6.66×10⁶，慢性牙周炎患者为4.47×10⁶，牙龈炎患者为1.78×10⁶。牙周炎组中古菌的患病率和古菌16S rRNA基因的平均数量高于牙龈炎组（P<0.05）。